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YEAST1 Sigma-Aldrich

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast



Related Categories Cloning and Expression, Core Bioreagents, General Reagents, Life Science Reagents for Cloning, Molecular Biology,
grade   for molecular biology
usage   1 kit sufficient for >100 standard transformations
shipped in   dry ice
storage temp.   −20°C



The Yeast Transformation Kit contains:
• Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
• Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
• Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
• Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
• Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g

Features and Benefits

• Easy and ready-to-use
• Requires as little as 10 ng of plasmid DNA
• Flexibility for any strain of yeast
• Sufficient for over 100 standard transformations

General description

Sigma’s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.


Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).


Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Kit component also available separately


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Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1 mL SDS D9156
Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g SDS Y1501

Kit component only


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Transformation Buffer 100 mL    
Plate Buffer 100 mL    
pRs316 Control plasmid 10 μg    
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
Precautionary statements 
UN 3316 9


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation


Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers


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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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