• USA Home
  • ZRB001906 - Anti-ATRX Antibody, clone 2A12, ZooMAb® Rabbit Monoclonal

ZRB001906 Sigma-Aldrich

Anti-ATRX Antibody, clone 2A12, ZooMAb® Rabbit Monoclonal

Green Alternative Antibody Enhanced Validation

recombinant, expressed in HEK 293 cells

Synonym: ATP-dependent helicase ATRX, EC:, Transcriptional regulator ATRX, X-linked helicase II, X-linked nuclear protein, XNP, Znf-HX



Related Categories 12 Principles Aligned Products, ATH-AZ, Alphabetical Index, Antibodies, Antibodies (Greener),
product line   ZooMAb® learn more
conjugate   unconjugated
clone   2A12, monoclonal
  recombinant monoclonal
biological source   rabbit (recombinant)
application(s)   immunocytochemistry: suitable using 1:100
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
  western blot: 1:1,000-1:4,000
species reactivity   mouse, human
mol wt   calculated mol wt 282.58 kDa
  observed mol wt ~280 kDa
enhanced validation   recombinant expression
Learn more about Antibody Enhanced Validation
form   lyophilized
shipped in   ambient
storage temp.   2-8°C
packaging   antibody small pack of 25 μL
antibody form   purified antibody
isotype   IgG
recombinant   expressed in HEK 293 cells
antibody product type   primary antibodies
greener alternative product characteristics   Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
UniProt accession no.   P46100
Gene Information   human ... ATRX; RAD54L; XH2(546)


General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 2A12 is a ZooMAb rabbit recombinant monoclonal antibody that detects Transcriptional regulator ATRX. It targets an epitope with in 141 amino acids from the C-terminal region.


GST/His-tagged recombinant fragment corresponding to 141 amino acids from the C-terminal region of human Transcriptional regulator ATRX (isoform 4).


Anti-ATRX, clone 2A12, ZooMAb, Cat. No. ZRB001906, is a recombinant Rabbit Monoclonal Antibody that specifically detects ATRX and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ATRX in human prostate tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ATRX in HeLa, A431, HUVEC, and NIH 3T3 cells.

Western Blot Analysis: A 1:1,000 - 1:4,000 dilution from a representative lot.

Target description

Transcriptional regulator ATRX (UniProt: P46100; also known as EC:, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein, XNP, Znf-HX, ATRX) is encoded by the ATRX (also known as RAD54L, XH2) gene (Gene ID: 546) in human. ATRX is ubiquitously distributed and is involved in transcriptional regulation and chromatin remodeling. ATRX has a PHD zinc finger motif and an ATPase/helicase domain of the SWI/SNF type. It facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. ATRX binds to DNA tandem repeat sequences in both telomeres and euchromatin and is shown to bind quadruplex structures in vitro. ATRX associates with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. It interacts with DAXX to form the chromatin remodeling complex ATRX:DAXX, which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres. ATRX is believed to be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. ATRX is phosphorylated at serine residues during mitosis and phosphorylation is most evident at pro-metaphase stage. Phosphorylation of ATRX is necessary for release of the protein from the nuclear matrix and that it may facilitate progression to mitosis. Dephosphorylation of ATRX seems to coincide with exit of the cells from M phase. Mutations in ATRX gene are known to cause severe psychomotor and mental retardation, facial dysmorphism, and urogenital abnormalities. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Berube, NG et al. (2000). Hum. Mol. Gen. 9(4):539-547).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?