• USA Home
  • 16044AST - Astec® CHIROBIOTIC® T2 Chiral HPLC Column

16044AST Supelco

Astec® CHIROBIOTIC® T2 Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 21.2 mm

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, Astec CHIROBIOTIC Chiral HPLC Columns (Macrocyclic Glycopeptides), Astec CHIROBIOTIC T2 Chiral HPLC Columns, CHIROBIOTIC T2, Chiral HPLC Columns,
description   HPLC column
packaging   pkg of 1 ea
application(s)   HPLC: suitable
L × I.D.   25 cm × 21.2 mm
matrix   high-purity silica gel particle platform
particle size   5 μm
pore size   200 Å
operating pH range   3.8 - 6.8
suitability   suitable for L63 per USP
separation technique   chiral


General description

CHIROBIOTIC® T and T2 have teicoplanin as the chiral selector. They offer unique selectivity for a number of classes of molecules, specifically underivatized α, β, γ and cyclic amino acids, N-derivatized amino acids, hydroxy-carboxylic acids, acidic compounds including carboxylic acids and phenols, small peptides, neutral aromatic analytes and cyclic aromatic and aliphatic amines. Separations normally obtained on a chiral crown ether or ligand exchange-type CSPs are also possible on the CHIROBIOTIC® T and T2, but in much simpler mobile phases, like water-alcohol. In addition, all of the known beta-blockers (amino alcohols), and dihydrocoumarins have been resolved. CHIROBIOTIC® T and T2 differ in their bonding chemistry and the pore size of the support particle, giving them different selectivity and preparative capacity.

• Bonded phase: Teicoplanin
• Operating pH range: 3.8 - 6.8
• Particle diameter: 5, 10 or 16 μm
• Pore size: 100 Å (CHIROBIOTIC® T) or 200 Å (CHIROBIOTIC® T2)

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Astec is a registered trademark of Sigma-Aldrich Co. LLC

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Milli-Q® Water Purification Solutions
Protocols & Articles


Advantages of Reversed phase for Preparative Chiral Separations

The use of reversed phase mode in preparative separations provides a number of benefits over normal phase methods. In addition to eliminating solubility issues often experienced in nonpolar normal ph...
Jennifer E. Claus
Reporter US Volume 27.5
Keywords: Chiral chromatography, Chromatography, Evaporation, High performance liquid chromatography, Purification, Separation, Solvents

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?