21568-U Supelco

Supelite DAX-8

jar of 5000 g

Synonym: XAD-8

  •  NACRES SB.52



Related Categories Adsorbent Media (Polymeric), Adsorbents (Resins), Amberlite, Amberlite & Amberchrom, Analytical/Chromatography,
Quality Level   100
form   solid
packaging   jar of 5000 g
application(s)   HPLC: suitable
  LPLC: suitable
  ion chromatography: suitable
matrix   acrylic ester
particle size   40-60 mesh
pore size   ~0.79 mL/g pore volume
  225 Å mean pore size
surface area   140 m2/g
density   1.09 g/mL at 25 °C (true wet)(lit.)
  1.23 g/mL at 25 °C (skeletal)(lit.)


General description

Supelite DAX-8 is a nonfunctionalised resin with strong hydrophobic organic matter endorsed to humic and fulvic acids. It is also referred as polymethylmethacrylate resin.


Supelite DAX-8 was used as an absorbent in:
• HPLC-SEC fractionation system
• column chromatography
• extraction procedure and these extracts were analysed using Cary Eclipse fluorescence spectrophotometer

Adsorbent resin with moderate polarity for compounds up to 150,000 MW. Most commonly used for adsorption of fulvic and humic acids, treatment of paper pulp mill waste, and removal of alcohols, surfactants, and colorants from samples.

Preparation Note

DAX-8 is a salt imbibed resin, which requires some preparation prior to use. In the protocol below, you may use methanol in place of ethanol.
• First, rinse the adsorbent resin with 2-3 bed volumes (BV) of distilled water.
• Soak overnight in 2 BV of 95% ethanol.
• After soaking, the resin is introduced into the column and rinsed with an additional 2 BV of 95% ethanol.
• Rinse with 2 BV of distilled water to displace the ethanol, followed by 1 BV of 4% (w/v) sodium hydroxide, 2 BV of distilled water, 1 BV 4% (v/v) hydrochloric acid, and finally by distilled water until the pH of the eluent becomes neutral.

Legal Information

Supelite is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

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