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  • 25445 - SPB®-1000 Capillary GC Column

25445 Supelco

SPB®-1000 Capillary GC Column

L × I.D. 30 m × 0.53 mm, df 0.50 μm

  •  eCl@ss 32119290



Related Categories All Gas Chromatography Columns, Analytical/Chromatography, Capillary GC Columns, Capillary GC Columns, by name, Gas Chromatography (GC),
material   fused silica
parameter   60-200 °C temperature (isothermal)
  60-220 °C temperature (programmed)
Beta value   265
df   0.50 μm
application(s)   gas chromatography (GC): suitable
L × I.D.   30 m × 0.53 mm
matrix active group   Bonded; acid-modified poly(ethylene glycol) phase
Agency/Method   meets requirements for USP G25 and G35
Featured Industry   Agriculture
Chemicals and Industrial Polymers
Cosmetics, Personal Care, and Cleaning Products
Food and Beverages
Forensics and Toxicology
column type   capillary polar


General description

Capillary GC column is also known as open tubular column. The carrier gas flows through the central aperture and is unrestricted throughout the length of the column.

Application: The incorporation of acid functional groups into the phase lends an acidic character to this column, useful for analyses of volatile acidic compounds. It offers great performance for analyses of glycols. It is the recommended column for ethylene glycol analysis.
USP Code: This column meets USP G25 and G35 requirements.
• Bonded
• Acid-modified poly(ethylene glycol)
Temp. Limits:
• 60 °C to 200 °C (isothermal) or 220 °C (programmed)

Other Notes

Complementary products:
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools

Legal Information

SPB is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the diameter of the column cage for Product number 25445, SPB-1000 Capillary GC Column?
For the 0.10-0.25mm ID columns, the cage is 6".The cage is 8" for all other columns.
Can I get an SBP-1000 capillary column with different dimensions than what you have as stock items?
Yes, we should be able to manufacture this column in most standard capillary column dimensions as custom items.
What is the phase of Product number 25445, SPB-1000 Capillary GC Column?
This is an improved version of the NUKOL phase and is recommended for Glycol analysis.This phase is a polyethylene glycol phase with the incorporation of acidic functional groups.This makes the phase useful for volatile acidic compounds.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles


ENVI-Carb Plus for the Extraction of Propylene Glycol and Ethylene Glycol from Water

Propylene glycol has a wide variety of applications, including uses as a solvent, emulsifier, and moisturizer in various chemical, food, and personal care products. Its toxicity to both humans and an...
Katherine K. Stenerson
Reporter US Volume 28.3
Keywords: Applications, Automotive, Chromatography, Environmental, Gas chromatography, Mass spectrometry, Methods, Sample preparations, Solid phase extractions, Solvents

GC Analysis of Glycols and Diols on SPB®-1000

From our library of Articles, Sigma-Aldrich presents GC Analysis of Glycols and Diols on SPB®-1000
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Purification, Size-exclusion chromatography

GC Analysis of Glycols and Diols

Glycol and diol compounds are used to solubilize active ingredients for use in many consumer products, including cosmetics, ballpoint pen inks, wood stains, and lacquers. They are also used as co-mon...
Katherine K. Stenerson, Michael D. Buchanan
Reporter US Volume 27.2
Keywords: Cosmetics, Gas chromatography, Help, Polymerization reactions, Reductions, Separation, Type

GC Column Selection Guide

The History of Supelco and the Capillary Column How to Choose a Capillary Column Additional Reading/Product Literature Column Phase Selection by Industry Column Phase Selection by Application Cross-R...
Keywords: Adsorption, Air Monitoring, Chemical industry, Chromatography, Derivatizations, Detection methods, Electron capture detector, Environmental, Gas chromatography, Herbicides, High performance liquid chromatography, Immobilization, Industries, Mass selective detector, Mass spectrometry, Metabolites, Nitrogen phosphorus detector, Pesticides, Pharmaceutical, Purification, Separation, Size-exclusion chromatography, Solid phase extractions, Solid phase microextractions, Solvents, Substitutions

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How to Choose a Capillary GC Column

An optimized chromatographic separation begins with the column. The selection of the proper capillary column for any application should be based on four significant factors: stationary phase, column ...
How to Choose a Capillary GC Column
Keywords: Chromatography, Condensations, Gas chromatography, Immobilization, Mass spectrometry, Pesticides, Separation, Size-exclusion chromatography, Solid phase microextractions, Solvents, Vaporization

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