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504955 Supelco

Discovery® C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

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Properties

Related Categories Analytical Column, 5 μm, 4.6 mm, Analytical/Chromatography, C18 HPLC Column, Discovery C18 HPLC columns for peptides, intermediate size molecules, HPLC Columns, by Phase,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   12% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
surface coverage   3 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   180 Å
surface area   200 m2/g
operating pH range   2 - 8
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Features and Benefits

• Excellent reproducibility
• Exceptional peak shape for basic and acidic analytes
• Stable, low-bleed LC-MS separations
• Separation of peptides and small proteins
• Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

A Case Study in SPE Method Development - Understanding the Dual Interaction Properties of Discovery DSC-SCX SPE Using Verapamil (and Metabolite) from Serum as a Test Example

A strong cation-exchange (SCX) SPE method was developed to selectively recover verapamil and its major metabolite, norverapamil from porcine serum for subsequent HPLC analysis. Initial experimentatio...
Reporter EU Volume 10
Keywords: Calorimetry, Cardiovascular, High performance liquid chromatography, Metabolites, Methods, Phase transitions, Protocols, Solid phase extractions, Solvents

Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method

The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the curren...
Keywords: High performance liquid chromatography, Sample preparations, Separation, Size-exclusion chromatography, Solid phase extractions

Extraction and Analysis of Lasix (Furosemide) in Horse Serum

The Office of Racing Commissioner’s (ORC) equine and human blood/urine testing programs play an important role towards ensuring the wagering public that drugs or other foreign substances are not admi...
Keywords: Agriculture, Calorimetry, Diuretics, Evaporation, High performance liquid chromatography, Mass spectrometry, Sample preparations, Solid phase extractions, Solvents

HPLC Analysis Report of Organic Acids on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Report of Organic Acids on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX
Keywords: Calorimetry, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Angiotensins on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Angiotensins on Discovery® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Aniline Homologs on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aniline Homologs on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Antibiotics on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibiotics on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt
Keywords: Calorimetry, Chromatography, Clinical, Forensic, High performance liquid chromatography, Pharmaceutical, Sample preparations, Separation, Solid phase extractions, Solvents

HPLC Analysis of Barbiturates on Discovery® C18, Unbuffered Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Barbiturates on Discovery® C18, Unbuffered Mobile Phase
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Barbiturates on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Barbiturates on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Carboxyamido-Methylated BSA Tryptic Digest on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamido-Methylated BSA Tryptic Digest on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Catecholamines on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Catecholamines on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Cylert (Pemoline) and Ritalin (Methylphenidate) on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cylert (Pemoline) and Ritalin (Methylphenidate) on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Cytochrome c Tryptic Digest on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cytochrome c Tryptic Digest on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Erectile Dysfunction Drugs on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Erectile Dysfunction Drugs on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Fluoxetine (Prozac®) on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fluoxetine (Prozac®) on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Fluroquinolone Antibiotics on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fluroquinolone Antibiotics on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18
Keywords: Calorimetry, Chromatography, Clinical, Diuretics, High performance liquid chromatography, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Furosemide and Indapamide on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Furosemide and Indapamide on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Organo-acid Pesticides on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Organo-acid Pesticides on Discovery® C18
Keywords: Chromatography, Environmental, Environmental Analysis, Herbicides, High performance liquid chromatography, Metabolites, Pesticides

HPLC Analysis of Paraben Preservatives on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Paraben Preservatives on Discovery® C18
Keywords: Chromatography, Cosmetics, High performance liquid chromatography

HPLC Analysis of Serotonin Synthetic Intermediates and Metabolites on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Serotonin Synthetic Intermediates and Metabolites on Discovery® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Hormones

HPLC Analysis of Substance P and Its Fragments on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Substance P and Its Fragments on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Sulfa Drugs on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sulfa Drugs on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18
Keywords: Calorimetry, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Urea, Carbamate, Triazine, Aniline Pesticides on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Urea, Carbamate, Triazine, Aniline Pesticides on Discovery® C18
Keywords: Chromatography, Environmental, Environmental Analysis, Herbicides, High performance liquid chromatography, Pesticides

HPLC Analysis of Vitamins, Fat Soluble (A and E) on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble (A and E) on Discovery® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamins, Water Soluble on Discovery® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Water Soluble on Discovery® C18
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

There are more than 100 antibiotics on the market today, and many more are in development. Resistance to antibiotics is a significant problem (1,2): an antibiotic that takes a decade to bring to mark...
Reporter EU Volume 14
Keywords: Antibiotics, Clinical, High performance liquid chromatography, Reversed-phase chromatography, Separation, Veterinary

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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