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505013 Supelco

Discovery® RP-AmideC16 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, Discovery HPLC Columns, Discovery RP-Amide C16, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   11% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
surface coverage   2.6 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical base material
matrix active group   amide, alkyl phase
particle size   5 μm
pore size   180 Å
surface area   200 m2/g
operating pH range   2 - 8
suitability   suitable for L60 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Application

Discovery RP-AmideC16 HPLC Column may be used for the separation of:
• Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
• Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
• Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
• Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery® RP-AmideC16 HPLC column may be used for the separation of:
• Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
• Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
• Hydrophilic bases by LC with principal component analysis (PCA).
• 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Features and Benefits

• Excellent retention and resolution of polar compounds
• Unique selectivity compared to C18
• Excellent peak shape and efficiency
• Less hydrophobic than C18 columns
• Compatible with 100% aqueous mobile phases
   

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Alkaloids on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Alkaloids on Discovery® RP-AmideC16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Amides on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Amides on Discovery® RP-Amide C16
Keywords: Analgesics, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Analgesics on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Analgesics on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Anti-Inflammatory Drugs on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Anti-Inflammatory Drugs on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihypertensive Drugs (β-Calcium Channel Blockers and ACE Inhibitor) on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihypertensive Drugs (β-Calcium Channel Blockers and ACE Inhibitor) on Discovery® RP-AmideC16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihypertensive and Diuretic Drugs on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihypertensive and Diuretic Drugs on Discovery® RP-Amide C16
Keywords: Antihypertensives, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antipyretics/Analgesics/Antifungals on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antipyretics/Analgesics/Antifungals on Discovery® RP-AmideC16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antitussive/Antihistamine/Antipyretic Mix on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antitussive/Antihistamine/Antipyretic Mix on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Bronchodilators Theophylline and Other Caffeine Metabolites in Serum on Discovery® RP-AmideC16 after SPE using Discovery® DSC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bronchodilators Theophylline and Other Caffeine Metabolites in Serum on Discovery® RP-AmideC16 after SPE using Discovery® DSC-18
Keywords: Calorimetry, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxyamido Methylated BSA Tryptic Digest on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Organophosphorus Pesticides on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Organophosphorus Pesticides on Discovery® RP-Amide C16
Keywords: Chromatography, Environmental, Environmental Analysis, Herbicides, High performance liquid chromatography, Pesticides

HPLC Analysis of Paraben Preservatives on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Paraben Preservatives on Discovery® RP-AmideC16
Keywords: Chromatography, Cosmetics, High performance liquid chromatography

HPLC Analysis of Sinus Medications on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sinus Medications on Discovery® RP-AmideC16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Tricyclic Antidepressants on Discovery® RP-AmideC16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tricyclic Antidepressants on Discovery® RP-AmideC16
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Vitamins, Water Soluble, on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Water Soluble, on Discovery® RP-Amide C16
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

HPLC Analysis of α-Hydroxy Aliphatic Acids on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of α-Hydroxy Aliphatic Acids on Discovery® RP-Amide C16
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of β-Lactam Antibiotics on Discovery® RP-Amide C16

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of β-Lactam Antibiotics on Discovery® RP-Amide C16
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Pharmaceutical

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

There are more than 100 antibiotics on the market today, and many more are in development. Resistance to antibiotics is a significant problem (1,2): an antibiotic that takes a decade to bring to mark...
Reporter EU Volume 14
Keywords: Antibiotics, Clinical, High performance liquid chromatography, Reversed-phase chromatography, Separation, Veterinary

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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Peer-Reviewed Papers
15

References

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