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50768-U Supelco

Ascentis® Express RP-Amide, 5 μm HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

  •  NACRES SB.52



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express RP-Amide Columns, Ascentis Express RP-Amide, 5μm HPLC Column, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
L × I.D.   5 cm × 4.6 mm
impurities   <5 ppm metals
matrix   Fused-Core® particle platform
matrix active group   amide, alkyl phase
particle size   5 μm
pore size   90 Å pore size
pH-range   2 - 9
suitability   suitable for L60 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase


General description

Ascentis Express 5 μm RP-Amide is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.6 μm thick porous shell and the highly uniform overall particle size of 5 μm. The one-step bonded, endcapped, amide-based, polar-embedded stationary phase of Ascentis Express 5 μm RP-Amide provides a stable, reversed-phase packing with decreased hydrophobic character. Ascentis Express RP-Amide can be used for basic, acidic, or neutral compounds with orthogonal selectivity from C18 or C8. The polar-embedded Ascentis Express 5 μm RP-Amide columns are compatible with 100% aqueous mobile phases without suffering from “phase collapse”.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

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