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50959-U Supelco

Ascentis® Express OH5, 2 μm UHPLC Column

2 μm particle size, L × I.D. 10 cm × 2.1 mm

  •  NACRES SB.52



Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express OH5 Columns, Ascentis Express OH5, 2μm UHPLC Columns, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   ≤60 °C temp. limit
  1000 bar max. pressure (14500 psi)
application(s)   UHPLC: suitable
L × I.D.   10 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   hydroxy, penta- phase
particle size   2 μm
pore size   90 Å pore size
pH-range   2 - 9
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)


General description

The Ascentis® Express 2μm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. This high performance material provides a UHPLC column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.


UHPLC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis® Express OH5 U/HPLC Columns

Ascentis® Express OH5 is a high-speed, high-performance liquid chromatography column based on Fused-Core® particle technology. The OH5 phase is a highly polar ligand that possesses 5 hydroxyl groups ...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Ion Exchange, Ligands, Mass spectrometry

Related Content

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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