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50994-U Supelco

BIOshell Glycan HPLC Columns

L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, BIOshell Glycan, 2.7 μm HPLC Columns, BIOshell HPLC Columns, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC More...
Quality Level   100
material   stainless steel hardware (column)
description   shell thickness (0.5 μm)
  solid core (1.7 μm)
feature   endcapped: no
analyte chemical class(es)   glycans
mfr. no.   BIOshell
parameter   1000 bar max. pressure (14500 psi)
  1000 bar pressure (14500 psi)
  60 °C max. temp.
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   15 cm × 2.1 mm
matrix   spherical silica particle platform
matrix active group   hydroxy, penta- phase
particle size   2.7 μm
pore size   90 Å
surface area   135 m2/g
operating pH range   2 - 9
suitability   suitable for L86 per USP
separation technique   hydrophilic interaction (HILIC)

Description

General description

BIOshell Glycan HPLC Columns are specifically engineered to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. The Fused-Core columns provide improved resolution when compared to conventional LC methods thus allowing more reliable quantitation and confident identification of glycans.

View the BIOshell Glycan HPLC Columns Flyer

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MSpngase fast asms

Comparison of PNGase F workflows for glycan release. The traditional workflow (top) involves multiple wash steps and an overnight digestion. With PNGase Fast (bottom), digestion is complete in 15 min...
Judy Boland, Amber Henry, Nicolas Caffarelli, Gordon Nicol, Jeffrey L. Turner, and Kevin Ray
Keywords: Aminations, Detergents, Digestions, Electrophoresis, High performance liquid chromatography, Mass spectrometry, PAGE, Reductions, Reductive aminations

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is a high-speed, high-performance liquid chromatography column based on a Fused-Core® particle design.
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Ligands, Separation

Empowering biochemists to analyze glycoproteins with exceptional reproducibility

Protein glycosylation is one of the most common and structurally diverse types of protein post translational modifications (PTM). The particular pattern of glycosylation as well as the location of th...
Keywords: Apoptosis, Chromatography, Glycosylations, Growth factors, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Normal-phase chromatography, Post translational modifications, Separation, Solid phase extractions

HPLC Analysis of Procainamide-Labeled Human IgG Glycans on BIOshell™ Glycan using HILIC-FLR

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Procainamide-Labeled Human IgG Glycans on BIOshell™ Glycan using HILIC-FLR
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR
Keywords: Chromatography, High performance liquid chromatography, Normal-phase chromatography

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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