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53345-U Supelco

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express Phenyl-Hexyl Columns, Ascentis Express Phenyl-Hexyl, 2.7μm HPLC Columns, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   10 cm × 3 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   phenylhexyl phase
particle size   2.7 μm
pore size   90 Å
pH-range   2 - 9
suitability   suitable for L11 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Can I use Ascentis Express on any type of HPLC system?
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Is there anything special I need to do to my HPLC system to use Ascentis Express?
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)
How can I measure my instrument bandwidth (IBW) and determine whatAscentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
What flow rate should I use with Ascentis® ExpressHPLC Columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Can Ascentis® Express HPLC Columns be used for LC-MS?
Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the pressure rating for Ascentis® Express Phenyl-Hexyl HPLC Columns?
This Ascentis Express column is stable to operating pressures up to 600 bar (9000 psi).
Can a 100% aqueous mobile phase be used with the Phenyl-Hexyl HPLC column?
The phenyl-hexyl phase is 100% aqueous compatible.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Analytical Standards of Boswellic Acids and Their Separation

The trees of the genus boswellia have been used in traditional medicine for thousands of years as an anti-inflammatory agent. Its resin is known as frankincense or olibanum and is still widely used i...
Matthias Nold, Hugh Cramer
AnaltyiX Volume 4, 2014
Keywords: Anti-inflammatory agents, High performance liquid chromatography, PAGE, Separation

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

The phenyl phase of the Ascentis® Express Phenyl-Hexyl column has unique reversed phase selectivity, particularly for polar aromatics and heterocyclic compounds, which arises from solute interaction ...
Keywords: Diffusion, High performance liquid chromatography

Column Selection Study for Analysis of Multiple Mycotoxins by LC-MS/MS

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Titan is a trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Separations Technology, Inc.
Emily R. Barrey1, Olga I. Shimelis1, and Christine Dumas2
1Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA, 2Sigma-Aldrich, St.Galen, Switzerland
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Horny Goat Weed Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Horny Goat Weed Extract Adulterated with Erectile Dysfunction Drugs on Ascentis® Express Phenyl-Hexyl
Keywords: Chromatography, Filtration, High performance liquid chromatography, Sample preparations, Vitamins

UHPLC Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl
Keywords: Chromatography, Vitamins

UHPLC Gradient Analysis of Boswellia Herbal Supplement on Ascentis® Express Phenyl-Hexyl

From our library of Articles, Sigma-Aldrich presents UHPLC Gradient Analysis of Boswellia Herbal Supplement on Ascentis® Express Phenyl-Hexyl
Keywords: Chromatography, Vitamins

UHPLC Gradient Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl

From our library of Articles, Sigma-Aldrich presents UHPLC Gradient Analysis of Boswellic Acids on Ascentis® Express Phenyl-Hexyl
Keywords: Chromatography, Vitamins

Related Content

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

Related Products

guard cartridge

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Description

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53526-U Ascentis® Express Phenyl-Hexyl, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

required but not provided

Product #

Description

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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