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53500-U Supelco

Ascentis® Express Guard Cartridge Holder

for use with Ascentis Express Guard Columns, pk of 1

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18 Columns, Ascentis Express C18, 2μm UHPLC Columns, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express C18, 5 μm HPLC Columns,
More...
Quality Level   100
packaging   pk of 1
application(s)   HPLC: suitable
Featured Industry   Food and Beverages
compatibility   for use with Ascentis Express Guard Columns

Description

General description

Guard cartridge not included with holder

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the part number for the ferrule on the Ascentis Express Guard Cartridge Holder?
The replacement ferrule for this holder is 51391-U, EXP Titanium Hybrid Ferrule.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Ascentis Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column. Th...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis ® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPL...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Ascentis ® Express HILIC U/HPLC Columns

HILIC chromatography is a variation of normal-phase chromatography with the distinction that one of the major components of the mobile phase is water. The high volatility of the mobile phase makes HI...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Normal-phase chromatography

Ascentis® Express ES-Cyano Reversed Phase U/HPLC Columns

Ascentis® Express ES-Cyano HPLC column is a high-speed, high-performance liquid chromatography column based on 90 Å Fused-Core® particles. The Fused-Core® particle provides a thin porous shell of hig...
Keywords: Chromatography, Diffusion, High performance liquid chromatography

Ascentis® Express F5 (pentafluorophenyl) U/HPLC Columns

Our Ascentis® Express F5 column brings together the highly efficient, robust Fused-Core® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Separation, Vitamins

Ascentis® Express OH5 U/HPLC Columns

Ascentis® Express OH5 is a high-speed, high-performance liquid chromatography column based on Fused-Core® particle technology. The OH5 phase is a highly polar ligand that possesses 5 hydroxyl groups ...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Ion Exchange, Ligands, Mass spectrometry

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

The phenyl phase of the Ascentis® Express Phenyl-Hexyl column has unique reversed phase selectivity, particularly for polar aromatics and heterocyclic compounds, which arises from solute interaction ...
Keywords: Diffusion, High performance liquid chromatography

Ascentis® Express RP-Amide Columns for HPLC and UHPLC

While the Ascentis® Express C8 and C18 provide classic reversed-phase selectivity, the RP-Amide phase provides alternate reversed-phase selectivity:
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

Monoclonal antibodies (mAbs) are widely manufactured by many biopharmaceutical companies to treat a myriad of diseases ranging from Alzheimer’s disease, Parkinson’s disease, ulcerative colitis, and m...
Keywords: Amplification, Chromatography, Diffusion, Diseases, High performance liquid chromatography, Mass spectrometry, Methylations, Microscopy, Oxidations, Pesticides, Pharmaceutical, Phosphorylations, Reversed-phase chromatography, Scanning electron microscopy, Solid phase peptide synthesis

Bath Salts in Urine

LC-MS Analysis of Illicit Bath Salts in Urine on Ascentis Express HILIC after Solid Phase Extraction (SPE) on Supel-Select SCX
Keywords: Chromatography, Diagnostic, Forensic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation, Solid phase extractions

HPLC Analysis of Synthetic (Spice) Cannabinoids

Synthetic cannabinoids (Spice) are a relatively new type of designer drug used as a pseudo-legal means to get a cannabis-type high. New synthetic cannabinoids are continually being introduced as supp...
Keywords: Diagnostic, Forensic, Separation

Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep

Clinical interest in vitamin D stems from its implications in an ever-widening range of human health conditions.1 This heightened interest has spawned the need for analytical strategies to assess an ...
Craig Aurand
Reporter US Vol 31.1
Keywords: Clinical, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions, Solvents, Vitamins

LC-MS Analysis of Vitamin D Metabolites

Vitamin D deficiency has become a topic of interest in recent publications.1-3 Vitamin D, along with calcium, promotes proper bone growth in children and aids in the prevention of osteoporosis in old...
Craig R. Aurand, David S. Bell
Reporter US Volume 29.2
Keywords: Clinical, Diagnostic, Diseases, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Separation, Vitamins

Online SPE/LC-MS Method for the Rapid Analysis of Thyroid Hormones in Plasma

The power of bonded phase selectivity in both sample prep and separation is demonstrated here. The Ascentis Express RP-Amide was shown to be a better trapping phase than conventional C8, and the Asce...
Xiaoning Lu, and David Bell
Reporter US, Volume 32.1
Keywords: Biological processes, Centrifugation, Chromatography, Clinical, Diseases, High performance liquid chromatography, Hormones, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Sample preparations, Separation, Solid phase extractions

Spice Cannabinoids in Plasma

LC-MS/MS Analysis of Synthetic (Spice) Cannabinoids from Plasma on Ascentis Express F5 after SPE using HybridSPE-Phospholipid
Keywords: Diagnostic, Forensic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase extractions, Solvents

Threading the Molecular Needle: The Importance of Particle Pore Diameter for Biomacromolecule Separations

Recently, the evolution of middle to large biomacromolecules such as peptides or proteins is apparent in the market of pharmaceutical drugs in addition to traditional active ingredients based on smal...
Keywords: Adsorption, Chromatography, Clinical, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Methylations, Oxidations, Pharmaceutical, Phosphorylations, Separation, Solid phase peptide synthesis

Related Content

Ascentis® Express Peptide ES-C18 U/HPLC Columns

The Ascentis® Express Peptide ES-C18 columns are best used with mobile phases that are mixtures of acetonitrile and water or methanol and water. Higher levels of the organic solvent component will ty...
Keywords: Buffers, Catalysis, Chromatography, Diffusion, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Separation

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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53537-U Ascentis® Express Peptide ES-C18, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea
53542-U Ascentis® Express Peptide ES-C18, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

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