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53577-U Supelco

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 7.5 cm × 3 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express F5 Columns, Ascentis Express F5, 2.7μm HPLC Column, F5, 2.7μm Columns,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   7.5 cm × 3 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   PFP (pentafluorophenyl) phase
particle size   2.7 μm
pore size   90 Å pore size
operating pH range   1 - 9
suitability   suitable for L43 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  reversed phase

Description

General description

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
What is special about Ascentis® Express F5 HPLC Columns?
Ascentis® Express F5 HPLC Columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Are guard columns available for Ascentis® Express F53.0 mm IDHPLC Columns?
Yes, we have guard columns available for product Ascentis® Express F5 3.0 mm IDHPLC Columns.Product 53500-U is the Ascentis® Express Guard Holder and Product 53597-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5 mm × I.D. 3.0 mm .
Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
Ascentis® Express F5 HPLC Columns HPLC columnsare capable of use on standard HPLC systems as well as UHPLC systems.These columnsare packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?
Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What flow rate should I use with Ascentis® Express F5 HPLC columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
Can Ascentis® Express F5 HPLC columns be used for LC-MS?
Yes, Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the pressure rating for Ascentis® Express F5 HPLC Columns?
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
What is the carbon load for Ascentis® Express F5 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Alternative Retention and Selectivity Using Fluorinated Stationary Phases

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Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
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Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis® Express F5 (pentafluorophenyl) U/HPLC Columns

Our Ascentis® Express F5 column brings together the highly efficient, robust Fused-Core® particle technology with F5 chemistry for the successful separation of polar and non-polar organic compounds.
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Benzodiazepines on Ascentis® Express Phenyl Hexyl HPLC Columns

The vast majority of UHPLC separations have been carried out with C18 columns in the classic reversed-phase (RP) mode; however, suppliers now offer many different phases. Although no one would disput...
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LC-MS Analysis of Vitamin D Metabolites

Vitamin D deficiency has become a topic of interest in recent publications.1-3 Vitamin D, along with calcium, promotes proper bone growth in children and aids in the prevention of osteoporosis in old...
Craig R. Aurand, David S. Bell
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Keywords: Clinical, Diagnostic, Diseases, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Separation, Vitamins

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Ascentis® Express U/HPLC Column Selection

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Peer-Reviewed Papers
15

References

Related Products

guard cartridge

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Description

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53597-U Ascentis® Express F5, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea

required but not provided

Product #

Description

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53500-U Ascentis® Express Guard Cartridge Holder, for use with Ascentis Express Guard Columns, pk of 1

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