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53822-U Supelco

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18 Columns, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Columns,
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   5 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å
operating pH   2 - 9
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Is there anything special I need to do to my HPLC system to use Ascentis Express?
Nothing special is required to use Ascentis Express HPLC columns.To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
Can I use Ascentis Express on any type of HPLC system?
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How can I measure my instrument bandwidth (IBW) and determine whatAscentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
What flow rate should I use with Ascentis® ExpressHPLC Columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Can Ascentis® Express HPLC Columns be used for LC-MS?
Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the carbon load for Ascentis® Express C18 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

Articles

Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 | Fast LC-MS-MS

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Keywords: Absorption, Cancer, Chromatography, Clinical, Diseases, Gene expression, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Sample preparations, Separation, Solid phase extractions, Vitamins

Ascentis Express C8 U/HPLC Columns

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Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis ® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPL...
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Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Steroid hormones are derivatives of cholesterol and play an important role in a large variety of organisms, as they can have direct control over the gene expression. 17β-estradiol (E2) controls the g...
Rudolf Köhling, Senior Scientist, LC/MS Applications
Analytix Volume 4, 2011, Article 4
Keywords: Clinical, Derivatizations, Environmental, Gene expression, High performance liquid chromatography, Hormones, Mass spectrometry, Metabolites, Pharmaceutical, Reversed-phase chromatography, Solvents

Derivatization and Separation of Aliphatic Amines

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

Fast Extraction and Low Level Detection of Chloramphenicol in Shrimp

QuEChERS Sample Preparation Using Z-Sep+ Sorbent and Analysis by Ascentis® Express C18 LC-MS/MS
Emily Barrey, Olga Shimelis, Michael Halpenny, Jennifer Claus
Reporter US Volume 32.1
Keywords: Antibiotics, Carcinogens, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Precipitation, Sample preparations

HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bisphenol A in Water on Ascentis® Express C18 after SPE using Supelclean™ ENVI-18
Keywords: Chromatography, Environmental, Environmental Analysis, High performance liquid chromatography, Reproduction, Solid phase extractions

HPLC Analysis of Lutein on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lutein on Ascentis® Express C18
Keywords: Chromatography, Cosmetics, High performance liquid chromatography, Pharmaceutical, Positron Emission Tomography, Sample preparations, Solid phase extractions, Vitamins

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid
Keywords: Antihypertensives, Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Sample preparations, Solid phase extractions

Higher Efficiency of Ascentis® Express 2.7 μm Compared to 3 μm Particles Gives Better Sensitivity

From our library of Articles, Sigma-Aldrich presents Higher Efficiency of Ascentis® Express 2.7 μm Compared to 3 μm Particles Gives Better Sensitivity
Keywords: Anti-depressants, Chromatography, High performance liquid chromatography, Pharmaceutical

Improved Recovery of Fungicide Carbendazim from Orange Juice

The fungicide carbendazim can sometimes be used to combat mold on citrus fruits including oranges. Recently, low residue levels of the fungicide were reported to be present in bulk orange juice impor...
Olga Shimelis and Emily Barrey
Reporter US Volume 30.2
Keywords: Chromatography, Fungicides, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations

Increase Bioanalytical Assay Speed via Phospholipid Removal using HybridSPE™-Precipitation Technology

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
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Increase Bioanalytical Assay Speed via Phospholipid Removal | HybridSPE

One of the primary concerns when analyzing small molecules in biological samples via LC-MS/MS is ion-suppression. Ion-suppression is caused by one or more interfering components or species that co-el...
Craig Aurand, An Trinh
Reporter US Volume 26.5
Keywords: Anticoagulants, Buffers, Centrifugation, Chromatography, Coagulants, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Reversed-phase chromatography, Size-exclusion chromatography, Solid phase extractions

LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of 25-Hydroxyvitam D3 and 25-Hydroxyvitamin D2 on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

LC/MS Analysis of Aflatoxin M1 in Milk on Ascentis® Express HILIC after SPE using Supel™ Tox AflaZea, Quantifying Picogram Concentrations

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Aflatoxin M1 in Milk on Ascentis® Express HILIC after SPE using Supel™ Tox AflaZea, Quantifying Picogram Concentrations
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Precipitation, Sample preparations, Separation, Solid phase extractions

LC/MS Analysis of Chloramphenicol in Shrimp on Ascentis® Express C18 after Dispersive SPE (QuEChERS) Cleanup using Supel™ QuE Z-Sep+

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Chloramphenicol in Shrimp on Ascentis® Express C18 after Dispersive SPE (QuEChERS) Cleanup using Supel™ QuE Z-Sep+
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions

LC/MS Analysis of Plasma Samples on Ascentis® Express C18 Showing Baseline Improvements after Phospholipid Removal using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Plasma Samples on Ascentis® Express C18 Showing Baseline Improvements after Phospholipid Removal using HybridSPE®-Phospholipid
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Precipitation, Sample preparations, Solid phase extractions

LC/MS/MS Analysis Using On-line Cartridges for the Removal of Phospholipids from Protein Precipitated Biological Fluid Samples

Phospholipids (PLs) are abundantly (at the mg/mL level) present in biological fluids such as: blood, plasma, serum, and cerebrospinal fluids,  among others. PLs are often co-extracted with a broad ra...
Keywords: Eliminations, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Precipitation, Sample preparations, Separation, Solid phase extractions

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel™ QuE PSA/C18 QuEChERS Cleanup
Keywords: Chromatography, Column chromatography, Fungicides, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Drugs in Plasma on Ascentis® Express C18 after Extraction using SPME LC Tips

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Drugs in Plasma on Ascentis® Express C18 after Extraction using SPME LC Tips
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation, Solid phase microextractions

LC/MS/MS Analysis of Steroids on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Steroids on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents

LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis® Express C18 after Extraction with BioSPME using C18 SPME LC Tips

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Testosterone in Human Serum on Ascentis® Express C18 after Extraction with BioSPME using C18 SPME LC Tips
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase microextractions

LC/MS/MS of Pesticides in Oranges on Ascentis® Express C18 after Sample Prep using Supel™ Que Z-Sep/C18 QuEChERS sorbent

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of Pesticides in Oranges on Ascentis® Express C18 after Sample Prep using Supel™ Que Z-Sep/C18 QuEChERS sorbent
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

Molecularly Imprinted Polymer SPE Increases Sensitivity for the Extraction of Clenbuterol from Urine

Clenbuterol (Figure 1) is a beta-agonist known for its growth-promoting properties in which use of the drug induces significant weight gain by increasing the proportion of muscle mass to fat. Althoug...
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Phospholipid Extractions for Cleanup or Enrichment

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Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk using SPE Cleanup and LC/MS Analysis

Aflatoxins are produced by fungi of the Aspergillus genus. Of several aflatoxin producing species, two are widely studied due to their importance to agriculture and medicine: Aspergillus flavus and A...
K. G. Espenschied, Emily Barrey, Michael Ye
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Keywords: Agriculture, Cancer, Carcinogens, Centrifugation, Eliminations, Evaporation, High performance liquid chromatography, Mass spectrometry, Metabolites, Precipitation, Sample preparations, Solid phase extractions

Rapid Determination of Protein Binding Affinity Using Solid Phase Microextraction

Determination of free circulating drug concentration is important in establishing pharmacokinetic activity. In most cases, drug-protein complexes could be formed which affect the active level of circ...
Emily R. Barrey, Senior Scientist, R&D and Craig Aurand, Innovation Manager, Advanced Analytical
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Serum Lipidomics Profiling | Ascentis Express Fused-Core C18 Column

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Supel-Select HLB SPE for Pharmaceutical Analysis

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Carmen T. SantasaniaAn Trinh andMati Sarker
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UHPLC/MS Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Vitamin K1 and Related Compound on Ascentis® Express C18
Keywords: Chromatography, Clinical, Mass spectrometry, Vitamins

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Ascentis® Express U/HPLC Column Selection

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

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Peer-Reviewed Papers
15

References

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