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53823-U Supelco

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18 Columns, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Columns,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
  600 bar max. pressure (9000 psi)
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   10 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å
operating pH   2 - 9
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis® Express C30 Compared to Ascentis® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Is there anything special I need to do to my HPLC system to use Ascentis Express?
Nothing special is required to use Ascentis Express HPLC columns.To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
Can I use Ascentis Express on any type of HPLC system?
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How can I measure my instrument bandwidth (IBW) and determine whatAscentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
What flow rate should I use with Ascentis® ExpressHPLC Columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Can Ascentis® Express HPLC Columns be used for LC-MS?
Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
What is the carbon load for Ascentis® Express C18 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is growing conc...
Emily Barrey, Olga Shimelis, K. G. Espenschied
Reporter US, Volume 32.2
Keywords: Antibiotics, High performance liquid chromatography, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions

Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 | Fast LC-MS-MS

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Keywords: Absorption, Cancer, Chromatography, Clinical, Diseases, Gene expression, High performance liquid chromatography, Indicators, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Precipitation, Sample preparations, Separation, Solid phase extractions, Vitamins

Analysis of Mycotoxins in Corn, Wheat, and Peanut

The Supel™ Tox AflaZea SPE cartridge was used to demonstrate the cleanup of corn meal, wheat meal, and raw peanut paste matrices prior to the analysis of Aflatoxin B1, B2, G1, and G2. In the procedur...
Keywords: Filtration, Fluorescence detector, High performance liquid chromatography, Purification, Sample preparations, Solid phase extractions

Ascentis Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column. Th...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis ® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPL...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma

Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demon...
Tracy L. Ascah, and Craig R. Aurand
Reporter US Volume 31.2
Keywords: Anticoagulants, Chromatography, Clinical, Forensic, Genetic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Phase transitions, Precipitation, Separation, Solid phase extractions, Solvents, Vitamins

Column Selection Study for Analysis of Multiple Mycotoxins by LC-MS/MS

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Titan is a trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Separations Technology, Inc.
Emily R. Barrey1, Olga I. Shimelis1, and Christine Dumas2
1Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA, 2Sigma-Aldrich, St.Galen, Switzerland
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Derivatization and Separation of Aliphatic Amines

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

Determination of Bisphenol A in Drinking Water

The level of bisphenol A (BPA) detected in foods and beverages has gained media attention over the last several years. Specifically, this interest is related to two types of containers:
Craig Aurand
Reporter US, Volume 30.1
Keywords: Derivatizations, Detection methods, Gas chromatography, High performance liquid chromatography, Sample preparations, Solid phase extractions

Fundamental Evaluation of the Stationary Phase Retention and Selectivity on the Ascentis®Express Biphenyl

While C18 alkyl-based phases remain the gold standard for reversed-phase liquid chromatography, stationary phases that invoke interactions in addition to hydrophobic partitioning afford the method de...
Daniel Shollenberger, Hugh Cramer, Dave Bell
Reporter US Volume 34.3
Keywords: Bacterial conjugations, Characterizations, Chromatography, Separation, Solvation

HPLC Analysis Report of Aflatoxins on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Report of Aflatoxins on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea
Keywords: Chromatography, High performance liquid chromatography, Pesticides, Sample preparations, Solid phase extractions

HPLC Analysis of Guaifenesin and Impurity A and B on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Guaifenesin and Impurity A and B on Ascentis® Express C18
Keywords: High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18
Keywords: Chromatography, Forensic, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)
Keywords: Chromatography, Clinical, High performance liquid chromatography, Separation

HPLC Analysis of Steroids on Ascentis® Express C18 with Methanolic Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® Express C18 with Methanolic Mobile Phase
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express C18
Keywords: Anti-depressants, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Undecenoic Acid in Antifungal Spray on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Undecenoic Acid in Antifungal Spray on Ascentis® Express C18
Keywords: Antifungals, Antimicrobials, Derivatizations, High performance liquid chromatography, Sample preparations

HPLC Determination of Free Undecenoic Acid in Antifungal Powder on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Determination of Free Undecenoic Acid in Antifungal Powder on Ascentis® Express C18
Keywords: Antifungals, Antimicrobials, Derivatizations, High performance liquid chromatography, Sample preparations

HPLC Determination of Total Undecenoic Acid in Antifungal Powder After the Complete Dissociation of Zinc Undecylenate on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Determination of Total Undecenoic Acid in Antifungal Powder After the Complete Dissociation of Zinc Undecylenate on Ascentis® Express C18
Keywords: Antifungals, Antimicrobials, Derivatizations, High performance liquid chromatography, Sample preparations

HPLC Separation of D-sphingosine and related compounds on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Separation of D-sphingosine and related compounds on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Separation

Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials

Sialic acids are N- or O-substituted derivatives of neuraminic acid. They are important because they affect bioavailability, function, stability, and metabolism of glycoproteins. Two forms of sialic ...
Xiaoning Lu, Isil Yasa, Ben Cutak, Kevin Ray, David S. Bell
Reporter US Volume 33.2
Keywords: High performance liquid chromatography, Metabolism, Separation, Solvents

LC/MS (TOF) Analysis of Cyanidin Glycosides from High Bush Blueberries on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Cyanidin Glycosides from High Bush Blueberries on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS (TOF) Analysis of Delphinidin Glycosides from High Bush Blueberries on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Delphinidin Glycosides from High Bush Blueberries on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS (TOF) Analysis of Malvinidin Glycosides from High Bush Blueberries on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Malvinidin Glycosides from High Bush Blueberries on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Peonidin Glycosides from High Bush Blueberries on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS (TOF) Analysis of Petunidin Glycosides from High Bush Blueberries on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Petunidin Glycosides from High Bush Blueberries on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column
Keywords: Anti-depressants, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Reversed-phase chromatography, Sample preparations

LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow
Keywords: Chromatography, High performance liquid chromatography, Vitamins

LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Mass spectrometry, Vitamins

LC/MS/MS Analysis of Steroid Hormones in Plasma on Ascentis® Express C18 after Sample Prep using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Steroid Hormones in Plasma on Ascentis® Express C18 after Sample Prep using HybridSPE®-Phospholipid
Keywords: Anticonvulsants, Chromatography, Clinical, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Precipitation, Separation, Solid phase extractions, Solvents

LC/MS/MS Analysis of Steroid Hormones in Plasma

HybridSPE®-Phospholipid plates provided excellent analyte recovery of these difficult compounds from plasma samples with subsequent LC/MS/MS analysis on Ascentis® Express Fused-Core® C18 columns. The...
Craig Aurand
Reporter US Volume 32.2
Keywords: Clinical, High performance liquid chromatography, Hormones, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Precipitation, Reductions, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Warfarin™ in Plasma on Ascentis® Express C18 after SPE using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Warfarin™ in Plasma on Ascentis® Express C18 after SPE using HybridSPE®-Phospholipid
Keywords: Anticoagulants, Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Reversed-phase chromatography, Solid phase extractions, Solvents

Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk using SPE Cleanup and LC/MS Analysis

Aflatoxins are produced by fungi of the Aspergillus genus. Of several aflatoxin producing species, two are widely studied due to their importance to agriculture and medicine: Aspergillus flavus and A...
K. G. Espenschied, Emily Barrey, Michael Ye
Reporter US Volume 33.1
Keywords: Agriculture, Cancer, Carcinogens, Centrifugation, Eliminations, Evaporation, High performance liquid chromatography, Mass spectrometry, Metabolites, Precipitation, Sample preparations, Solid phase extractions

Rapid Screening of Pharmaceutical Compounds | Fast HPLC

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its ability to ...
Hillel Brandes
Reporter EU Volume 30
Keywords: Anti-depressants, Chromatography, Diffusion, High performance liquid chromatography, Mass spectrometry, PAGE, Pharmaceutical, Reductions, Separation

Serum Lipidomics Profiling | Ascentis Express Fused-Core C18 Column

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Reporter US Volume 29.5
Keywords: Analytical Chemistry, Chromatography, Diffusion, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Separation

UHPLC Analysis of Catecholamines on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Catecholamines on Ascentis® Express F5
Keywords: Chromatography, Clinical

Related Content

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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