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53982-U Supelco

Ascentis® Express C18, 2.7 Micron Capillary HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 75 μm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, Ascentis Express C18 Columns, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Capillary Columns,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
parameter   60 °C temp. range
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   5 cm × 75 μm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å pore size
operating pH   2 - 9
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is special about Ascentis Express?
Ascentis Express columns provide a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis Express provides the benefits of sub-2 μm particles but at much lower backpressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the carbon load for Ascentis® Express C18 HPLC Columns?
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
What is the pressure rating for75 μmI.D. Ascentis®Express Capillary HPLC columns?
The 75 μmI.D. Ascentis® Express Capillary HPLCcolumnsarestable to operating pressures up to 8500 psi.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Ascentis Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column. Th...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µm UHPLC columns are...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Ascentis ® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method. The Fused-Core® particle technology of Ascentis® Express C18 enables high speed and high resolution separations on HPL...
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

Related Content

Ascentis® Express U/HPLC Column Selection

The column selectivity has the highest influence on resolution in chromatography. Therefore, the selection of the best suitable column chemistry for the target analytes is an important selection para...
Keywords: Chromatography, High performance liquid chromatography, Separation

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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Peer-Reviewed Papers
15

References

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