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54096-U Supelco

Supelclean ENVI-Carb/NH2 SPE Tube

bed B 500 mg (Supelclean LC-NH2), bed A 500 mg (Supelclean ENVI-Carb), volume 20 mL, pk of 200



Related Categories Analytical/Chromatography, Sample Preparation & Purification, Solid Phase Extraction (SPE), Supelclean ENVI and Supelclean SPE Cartridges, Supelclean ENVI-Carb/NH2 SPE Products More...
composition   bed A, 500 mg (Supelclean ENVI-Carb)
  bed B, 500 mg (Supelclean LC-NH2)
packaging   pk of 200
volume   20 mL
matrix active group   aminopropyl phase
  carbon phase
mode of chromatography   ion exchange
  reversed phase


General description

Retention Mechanism: Reversed-phase and anion-exchange
Sample Matrix Compatibility: Organic or aqueous solutions

• Dual layer SPE tube that contains both Supelclean ENVI-Carb (upper layer) & LC-NH2 (lower layer) SPE sorbents (separated by PE frit)
• Developed to offer superior clean up when conducting multi-residue pesticide analysis from food (e.g. agricultural products, meats, etc.).
• ENVI-carb has a strong affinity towards planar molecules, and can isolate/remove pigments (e.g., chlorophyll and carotinoids) and sterols commonly present in foods and natural products
• Supelclean LC-NH2 is an aminopropyl phase that retains fatty acids, organic acids, and some polar pigments and sugars common in food matrices

Legal Information

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


Solid Phase Extraction: Ion Exchange Methodology

In order for electrostatic retention to occur, both analyte and sorbent functional groups must be in their ionized form. This is done through strict pH control of the sample matrix. For basic analyte...
Keywords: Agriculture, Buffers, Herbicides, Ion Exchange, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Normal Phase Methodology

In order for polar retention to occur between the sorbent and the sample, the analyte must be introduced to the SPE device in a non-polar sample or mobile phase environment. Therefore, typical sample...
Keywords: Cosmetics, Fractionation, Gas chromatography, Separation, Solid phase extractions, Solvents, Sonication

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. In other words, it may be difficult for a reversed-phase method or bonded ch...
Keywords: Environmental, Evaporation, Gas chromatography, High performance liquid chromatography, Homogenization, Metabolites, Phase transitions, Solid phase extractions, Solvents

Related Content

SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Fundamentals and Recent Developments [Webinar]

Solid Phase Extraction (SPE) has become a popular technique for sample preparation due to its convenience, effectiveness, relatively low solvent usage and versatility. The focus of this webinar is on...
Keywords: Sample preparations, Solid phase extractions

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