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54181-U Supelco

Supel-Select HLB SPE Tube

bed wt. 30 mg, volume 1 mL, pk of 100

  •  NACRES NB.21

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Properties

Related Categories Analytical/Chromatography, HLB SPE Tubes and 96-well Plates, Sample Preparation & Purification, Solid Phase Extraction (SPE), Supel-Select SPE More...
Quality Level   100
material   PE frit (20 μm)
  polypropylene hardware
quality   MS friendly
composition   bed wt., 30 mg
packaging   pk of 100
volume   1 mL
matrix   Hydrophilic modified styrene polymer particle platform
matrix active group   polystyrene phase
particle size   50-70 μm
pore size   80-200 Å pore size
surface area   160-420 m2/g
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)

• Hydrophilic modified styrene polymer
• Developed for the extraction of a highly broad range of compounds from aqueous samples
• Retention predominately based on reversed-phase; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
• Examples of more polar compounds that are retained and recovered include (but not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).

Application

Supel-Select HLB SPE Tube may be used as an extraction cartridge for the solid phase extraction and analysis of caffeine and acetaminophen in amniotic fluid using liquid chromatography coupled to tandem mass spectrometry.

Features and Benefits

• Excellent sample prep performance at a lower price
• Amenable to generic methodology - save time, money, and headache during method development
• Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
• Resistance to overdrying allows for more robust methdology
• Low UV and MS extractables for lower background and greater sensitivity
• Stringent production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Sample preparations, Solid phase extractions

LC/MS Analysis of Glyphosate in Oatmeal on ApHera™ NH2 after SPE using Supel™ Select HLB

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Glyphosate in Oatmeal on ApHera™ NH2 after SPE using Supel™ Select HLB
Keywords: Absorption, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Separation, Solid phase extractions

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate is one of the most commonly used herbicides in the world. Its usage has significantly increased after the introduction of genetically modified glyphosate tolerant crops such as corn, soybe...
Olga I. Shimelis, Principal R&D Scientist
Reporter US Volume 34.4
Keywords: Absorption, Centrifugation, Derivatizations, Herbicides, High performance liquid chromatography, Mass spectrometry, Metabolites, Pesticides, Phase transitions, Sample preparations, Solid phase extractions

Phospholipid Extractions for Cleanup or Enrichment

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. Santasania, An Trinh, and Mati Sarker
Reporter Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. In other words, it may be difficult for a reversed-phase method or bonded ch...
Keywords: Environmental, Evaporation, Gas chromatography, High performance liquid chromatography, Homogenization, Metabolites, Phase transitions, Solid phase extractions, Solvents

Supel-Select HLB SPE for Pharmaceutical Analysis

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. SantasaniaAn Trinh andMati Sarker
Reporter US Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Supel™-Select Polymeric SPE

Supel-Select SPE phases are ideal for the solid phase extraction of a broad range of compounds from aqueous samples. While reversed-phase interactions dominate retention on the Supel-Select HLB, and ...
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase extractions

Related Content

SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Fundamentals and Recent Developments [Webinar]

Solid Phase Extraction (SPE) has become a popular technique for sample preparation due to its convenience, effectiveness, relatively low solvent usage and versatility. The focus of this webinar is on...
Keywords: Sample preparations, Solid phase extractions

Supel™-Select Polymeric SPE [Product Overview Video]

Supel-Select HLB and Ion-Exchange phases are ideal for efficient and highly reproducible solid phase extraction of a broad range of compounds from aqueous samples. Their use covers a range of applica...
Keywords: Solid phase extractions

Peer-Reviewed Papers
15

References

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