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  • 55465-U - Discovery® Glycan SPE Tube

55465-U Supelco

Discovery® Glycan SPE Tube

bed wt. 50 mg, volume size 1 mL, pk of 108

  •  NACRES NB.21



Related Categories Analytical/Chromatography, Discovery Glycan SPE Products, Discovery SPE Tubes, Sample Preparation & Purification, Solid Phase Extraction (SPE) More...
Quality Level   100
material   PE frit
composition   bed wt., 50 mg
packaging   pk of 108
volume size   1 mL
impurities   <5% Water content
matrix   polyamide resin base material
matrix active group   amide, poly- phase
particle size   50-160 μm
pH-range   4.5 - 7.5 (surface pH)
bulk density   0.2‑0.3 g/mL
separation technique   reversed phase


General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous or methanolic solutions

• Polyamide Resin: Particle Size: 50-160 μm, Surf pH: 4.5-7.5, Density: 0.2-0.3 cm3/g, Water Content: < 5 %
• Useful for extracting gylcans from aqueous solutions.
• Used to adsorb polar compounds (-OH groups, esp. phenolic compounds) from aqueous or methanolic solutions under the reversed-phase mechanism through strong hydrogen bonding between compound hydroxyl groups and amide groups of the resin
• Also may be used for the extraction of tannins, chlorophyll, humic acid, pharmacologically active terpenoids, flavanoids, gallic acid, catechol A, protocatechuic acid, phloroglucinol, aromatic carboxylic acids, and nitroaromatic compounds
• Irreversibly retains quinones.

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

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Certificate of Analysis (COA)

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Protocols & Articles


A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MSpngase fast asms

Comparison of PNGase F workflows for glycan release. The traditional workflow (top) involves multiple wash steps and an overnight digestion. With PNGase Fast (bottom), digestion is complete in 15 min...
Judy Boland, Amber Henry, Nicolas Caffarelli, Gordon Nicol, Jeffrey L. Turner, and Kevin Ray
Keywords: Aminations, Detergents, Digestions, Electrophoresis, High performance liquid chromatography, Mass spectrometry, PAGE, Reductions, Reductive aminations

Empowering biochemists to analyze glycoproteins with exceptional reproducibility

Protein glycosylation is one of the most common and structurally diverse types of protein post translational modifications (PTM). The particular pattern of glycosylation as well as the location of th...
Keywords: Apoptosis, Chromatography, Glycosylations, Growth factors, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Normal-phase chromatography, Post translational modifications, Separation, Solid phase extractions

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. In other words, it may be difficult for a reversed-phase method or bonded ch...
Keywords: Environmental, Evaporation, Gas chromatography, High performance liquid chromatography, Homogenization, Metabolites, Phase transitions, Solid phase extractions, Solvents

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SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Peer-Reviewed Papers


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