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56100AST Supelco

apHera C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 2 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, apHera C18 Reversed-phase Columns, apHera Polymeric HPLC columns, high pH applications More...
Quality Level   100
packaging   pkg of 1 ea
mfr. no.   apHera
parameter   147 bar pressure (2133 psi)
  4-60 °C temperature
application(s)   HPLC: suitable
L × I.D.   15 cm × 2 mm
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   300 Å
suitability   suitable for L67 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

apHera reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These columns equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features is logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.

Features of apHera Reversed Phase Columns
• Stable vinyl copolymer base
• Stable pH range 2-12
• 300 angstrom pore size
• Stable in all organic solvents
• Expanded applications: peptides, proteins
• Efficient separation of any basic substance
• Amenability to washing and alkaline solutions

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents

UHPLC Analysis of Antihistamines on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Antihistamines on Ascentis® Express C18
Keywords: Chromatography, Pharmaceutical

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56129AST apHera C18 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 2 mm

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