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56400AST Supelco

apHera NH2 HPLC Column

5 μm particle size, L × I.D. 15 cm × 2 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Amino HPLC Columns, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC Columns, by Phase, HPLC/UHPLC,
Quality Level   100
packaging   pkg of 1 ea
mfr. no.   apHera
parameter   147 bar pressure (2133 psi)
  4-50 °C temperature
application(s)   HPLC: suitable
L × I.D.   15 cm × 2 mm
matrix active group   amino phase
particle size   5 μm
pore size   300 Å
suitability   suitable for L82 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  normal phase


General description

apHera amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera Columns
• High efficiency for carbohydrate analysis
• Ideal for basic conditions/amphoteric detection
• High selectivity mono- to oligosaccharides
• pH range 2-13
• 300 angstrom pore size
• Stable, predictable retention
• Long column life

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

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Certificate of Analysis (COA)

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Protocols & Articles


LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2, 2 mm I.D. Column
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Mass spectrometry detection

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Peer-Reviewed Papers


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56429AST apHera NH2 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 2 mm

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