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56401AST Supelco

apHera NH2 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Amino HPLC Columns, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC Columns, by Phase, HPLC/UHPLC,
Quality Level   100
packaging   pkg of 1 ea
mfr. no.   apHera
parameter   147 bar pressure (2133 psi)
  4-50 °C temperature
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
matrix active group   amino phase
particle size   5 μm
pore size   300 Å
suitability   suitable for L82 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  normal phase

Description

General description

apHera amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera Columns
• High efficiency for carbohydrate analysis
• Ideal for basic conditions/amphoteric detection
• High selectivity mono- to oligosaccharides
• pH range 2-13
• 300 angstrom pore size
• Stable, predictable retention
• Long column life

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Simple Underivatized Sugars on apHera™ NH2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Simple Underivatized Sugars on apHera™ NH2
Keywords: Chromatography, High performance liquid chromatography

Improved Analysis of Simple Sugars Using apHera™ NH2 HPLC Columns

Chromatographic analysis of simple sugars is important to the food and beverage, pharmaceutical and biotech industries. Effective separations of sugars can be challenging because these compounds are ...
Hugh M. Cramer, Ric Cone
Reporter US Volume 25.2
Keywords: Applications, Chromatography, High performance liquid chromatography, Industries, Ligands, Liquid chromatography mass spectrometry, Mass spectrometry, Normal-phase chromatography, Pharmaceutical, Phase transitions, Separation, Type

LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Glyphosate and Metabolites on apHera™ NH2
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Mass spectrometry detection

LC/MS Analysis of Glyphosate in Oatmeal on ApHera™ NH2 after SPE using Supel™ Select HLB

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Glyphosate in Oatmeal on ApHera™ NH2 after SPE using Supel™ Select HLB
Keywords: Absorption, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Separation, Solid phase extractions

LC/MS Analysis of Glyphosate in Wheat Flour on ApHera™ NH2

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Glyphosate in Wheat Flour on ApHera™ NH2
Keywords: Adsorption, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Separation

LC/MS/MS Method for Determination of Glyphosate, AMPA, and Glufosinate in Cereals

Glyphosate is one of the most commonly used herbicides in the world. Its usage has significantly increased after the introduction of genetically modified glyphosate tolerant crops such as corn, soybe...
Olga I. Shimelis, Principal R&D Scientist
Reporter US Volume 34.4
Keywords: Absorption, Centrifugation, Derivatizations, Herbicides, High performance liquid chromatography, Mass spectrometry, Metabolites, Pesticides, Phase transitions, Sample preparations, Solid phase extractions

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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56430AST apHera NH2 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 4.6 mm

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