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  • 565309-U - Ascentis® RP-Amide HPLC Column

565309-U Supelco

Ascentis® RP-Amide HPLC Column

3 μm particle size, L × I.D. 5 cm × 1 mm

  •  eCl@ss 32110501



Related Categories Analytical column, 3 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis RP-Amide, HPLC,
feature   endcapped
extent of labeling   19.5% Carbon loading
parameter   ≤70 °C temp. range
application(s)   HPLC: suitable
L × I.D.   5 cm × 1 mm
surface coverage   2.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   amide, alkyl phase
particle size   3 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for L60 per USP
mode of chromatography   reversed phase


Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

LC-MS Grade Solvents and Reagents

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles

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