• USA Home
  • 565323-U - Ascentis® RP-Amide HPLC Column

EMAIL THIS PAGE TO A FRIEND
565323-U Supelco

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

Purchase

Properties

Related Categories Analytical column, 5 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis RP-Amide, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   19.5% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   5 cm × 4.6 mm
surface coverage   2.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   amide, alkyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for L60 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Alternative Selectivity of Amide-based Embedded Polar Group HPLC Stationary Phases – Role of Hydrogen Bonding

High-performance liquid chromatographic (HPLC) method development often commences using alkyl (C8, C18) stationary phases. The method development scientist will commonly adjust the mobile phase organ...
Dave Bell
Reporter EU Volume 24
Keywords: Atomic absorption spectroscopy, High performance liquid chromatography, Ligands, Separation, Solvation

HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, con...
Jacinth A. M. McKenzie, Wayne K. Way
Reporter EU Volume 26
Keywords: Absorption, Applications, Biochemistry, Cancer, Cosmetics, Degradations, Environmental, Fluorinations, Forensic, Halogenations, Help, High performance liquid chromatography, Mass spectrometry, Metabolites, Methods, Oxidations, Pharmaceutical, Separation, Substitutions

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

Related Products

guard cartridge

Product #

Description

Add to Cart

565370-U Ascentis® RP-Amide Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
565371-U Ascentis® RP-Amide Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

related product

Product #

Description

Add to Cart

59660-U Supelguard Guard Cartridge Holder, Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?