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565324-U Supelco

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical column, 5 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis RP-Amide, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   19.5% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
surface coverage   2.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   amide, alkyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for L60 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

Recommended products

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Alternative Selectivity of Amide-based Embedded Polar Group HPLC Stationary Phases – Role of Hydrogen Bonding

High-performance liquid chromatographic (HPLC) method development often commences using alkyl (C8, C18) stationary phases. The method development scientist will commonly adjust the mobile phase organ...
Dave Bell
Reporter EU Volume 24
Keywords: Atomic absorption spectroscopy, High performance liquid chromatography, Ligands, Separation, Solvation

HPLC Analysis of Anaesthetic Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Anaesthetic Drugs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Angiotensins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Angiotensins on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide (Formate Mobile Phase)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide (Formate Mobile Phase)
Keywords: Antifungals, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase
Keywords: Antibiotics, Antifungals, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® RP-Amide
Keywords: Antibiotics, Antimicrobials, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Anticholinergic Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Anticholinergic Drugs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihypertensive Drugs on Ascentis® RP-Amide (UV, 220 nm)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihypertensive Drugs on Ascentis® RP-Amide (UV, 220 nm)
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihypertensive Drugs on Ascentis® RP-Amide (UV, 254 nm)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihypertensive Drugs on Ascentis® RP-Amide (UV, 254 nm)
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antipsychotic Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antipsychotic Drugs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antiretroviral Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antiretroviral Drugs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Aromatics on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aromatics on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Benzoic Acid Deriviatives on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzoic Acid Deriviatives on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Benzoic Acid and Related Compounds on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzoic Acid and Related Compounds on Ascentis® RP-Amide
Keywords: Chromatography, Cosmetics, High performance liquid chromatography

HPLC Analysis of Caffeine and Metabolites on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Caffeine and Metabolites on Ascentis® RP-Amide
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Calcium Channel Blockers on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Calcium Channel Blockers on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Carotene Compounds on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carotene Compounds on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins

HPLC Analysis of Catechins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Catechins on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Vitamins

HPLC Analysis of Catechols and Resorcinols on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Catechols and Resorcinols on Ascentis® RP-Amide
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Cephalsporin Antibiotics on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cephalsporin Antibiotics on Ascentis® RP-Amide
Keywords: Antibiotics, Antimicrobials, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Codeine and Metabolite on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Codeine and Metabolite on Ascentis® RP-Amide
Keywords: Analgesics, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Cold Remedy Ingredients on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cold Remedy Ingredients on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fatty Acids on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fatty Acids on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Flavonoids on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Flavonoids on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of NSAIDs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of NSAIDs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Peptides on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Statins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Statins on Ascentis® RP-Amide
Keywords: Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 60:40)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 60:40)
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Vitamins, Water Soluble, on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Water Soluble, on Ascentis® RP-Amide
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

LC/MS Analysis of Cannabinoids on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Cannabinoids on Ascentis® RP-Amide
Keywords: Analgesics, Chromatography, High performance liquid chromatography, Mass spectrometry, Pharmaceutical

LC/MS Analysis of Sudan Red Dyes on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Sudan Red Dyes on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry

Mass Spectral Column Bleed in Nitrogen-Containing Polar-Embedded HPLC Stationary Phases

Liquid chromatography-mass spectrometry has become one of the most important tools in the analytical chemistry laboratory over the past decade. The structural information obtainable along with sensit...
Carmen T. Santasania, David S. Bell
Reporter EU Volume 16
Keywords: Analytical Chemistry, Chromatography, Environmental, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical

Reference Standards for Analyzing Polyphenol Catechins

Catechins have become a hot topic in today’s health conscious world. Current research suggests that catechins aid health by:
James S. Walbridge & Kathleen Kiefer
Reporter US Vol 25.4
Keywords: Anti-inflammatory agents, Antivirals, Cancer, Carcinogens, Degradations, High performance liquid chromatography, Inflammation, Mass spectrometry, Pharmaceutical, Respiratory

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Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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