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565325-U Supelco

Ascentis® RP-Amide HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Accessories for DSD-DNPH sampling, Air Monitoring, Analytical column, 5 micron, Analytical/Chromatography, Ascentis HPLC Columns,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   19.5% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
surface coverage   2.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   amide, alkyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for L60 per USP
Featured Industry   Environmental
Food and Beverages
separation technique   reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Features and Benefits

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
When are additional phases expected?
Additional phases are being developed.The best way to track new products is to visitAscentis Express for the latest updates.
Can I use Ascentis Express on a UHPLC system?
Yes.Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.These benefits include the capability of providing fast HPLC and higher resolution chromatography.The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Columnbe stored in?
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

EPA Method 8315: (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents EPA Method 8315: (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

US EPA Method 8315 (Solid Waste Carbonyls by HPLC-UV) on Ascentis® RP Amide

From our library of Articles, Sigma-Aldrich presents US EPA Method 8315 (Solid Waste Carbonyls by HPLC-UV) on Ascentis® RP Amide
Keywords: High performance liquid chromatography

US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents US EPA Method 8315 (Solid Waste/Ground Water Carbonyls by HPLC-UV) on Ascentis® C18
Keywords: High performance liquid chromatography

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Peer-Reviewed Papers
15

References

Related Products

guard cartridge

Product #

Description

Add to Cart

565370-U Ascentis® RP-Amide Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
565371-U Ascentis® RP-Amide Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

related product

Product #

Description

Add to Cart

59660-U Supelguard Guard Cartridge Holder, Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)

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