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  • 567523-U - Discovery® HS F5 HPLC Column

567523-U Supelco

Discovery® HS F5 HPLC Column

5 μm particle size, L × I.D. 25 cm × 21.2 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Analytical/Chromatography, Discovery HPLC Columns, Discovery HS F5, Discovery HS F5 Prep Columns, Discovery Semi-Prep and Preparative HPLC Columns,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   12% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 21.2 mm
surface coverage   4 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical particle platform
matrix active group   PFP (pentafluorophenyl) phase
particle size   5 μm
pore size   120 Å
surface area   300 m2/g
operating pH range   2 - 8
suitability   suitable for L43 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  reversed phase


General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

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Protocols & Articles

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