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568222-U Supelco

Discovery® BIO Wide Pore C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories 5 micron Discovery BIO Wide Pore (300) C18 Columns, 5 micron Discovery Wide Pore C18 HPLC Columns for proteins Columns, Analytical/Chromatography, C18 HPLC Column, Discovery BIO,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   9.2% Carbon loading
parameter   0-70 °C temperature
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
surface coverage   3.6 μmol/m2
matrix   silica particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   300 Å
surface area   100 m2/g
suitability   suitable for L1 per USP
separation technique   reversed phase

Description

General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

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Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (PFPA Mobile Phase Additive)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (PFPA Mobile Phase Additive)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 0-16 Min. Window
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 28-38 Min. Window

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18, 28-38 Min. Window
Keywords: Chromatography, High performance liquid chromatography

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Reporter US Volume 27.5
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568273-U Discovery® BIO Wide Pore C18 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit

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