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569226-U Supelco

Discovery® C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 21.2 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, C18 HPLC Column, Discovery C18 HPLC columns for peptides, intermediate size molecules, Discovery C18 Prep column, 5 μm, 21.2 mm, Discovery Semi-Prep and Preparative HPLC Columns,
Quality Level   100
feature   endcapped
mfr. no.   Discovery
extent of labeling   12% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 21.2 mm
surface coverage   3 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   180 Å
surface area   200 m2/g
operating pH range   2 - 8
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Features and Benefits

• Excellent reproducibility
• Exceptional peak shape for basic and acidic analytes
• Stable, low-bleed LC-MS separations
• Separation of peptides and small proteins
• Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

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Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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