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575662-U Supelco

Supel Select HLB SPE 96-well Plate

bed wt. 60 mg/well, pk of 1

Synonym: 60 mg/well

  •  NACRES NB.21

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Properties

Related Categories Analytical/Chromatography, HLB SPE Tubes and 96-well Plates, Sample Preparation & Purification, Solid Phase Extraction (SPE), Supel-Select SPE More...
Quality Level   100
material   PE frit (20 μm)
  polypropylene hardware
quality   MS friendly
composition   bed wt., 60 mg/well
packaging   pk of 1
matrix   Hydrophilic modified styrene polymer particle platform
matrix active group   polystyrene phase
particle size   50-70 μm
pore size   80-200 Å pore size
surface area   160-420 m2/g
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

Retention Mechanism: Reversed-phase
Sample Matrix Compatibility: Aqueous solutions (biological fluids, water)

• Hydrophilic modified styrene polymer
• Developed for the extraction of a highly broad range of compounds from aqueous samples
• Retention predominately based on reversed-phase; however, because the phase is hydrophilic modified, it is amenable to polar compounds as well.
• Examples of more polar compounds that are retained and recovered include (but not limited to): pyridoxine (logPo/w -0.56), riboflavin (logPo/w -2.02), biotin (logPo/w 0.11).

Features and Benefits

• Excellent sample prep performance at a lower price
• Amenable to generic methodology - save time, money, and headache during method development
• Greater capacity allows for smaller bed weights = smaller elution volumes = time savings in sample processing
• Resistance to overdrying allows for more robust methdology
• Low UV and MS extractables for lower background and greater sensitivity
• Stringer production and QC guidelines offer greater lot-to-lot, tube-to-tube, and well-to-well consistency for improved accuracy and precision

Legal Information

Supel is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

LC/MS (TOF) Analysis of Opioid Drugs and Glucuronide Metabolites in Urine on Ascentis® Express HILIC after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Opioid Drugs and Glucuronide Metabolites in Urine on Ascentis® Express HILIC after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Metabolites, Sample preparations, Solid phase extractions

LC/MS Analysis of Opioid Glucuronide Metabolites in Urine on Ascentis® Express F5 after Solid Phase Extraction (SPE) using Supel™-Select HLB

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Opioid Glucuronide Metabolites in Urine on Ascentis® Express F5 after Solid Phase Extraction (SPE) using Supel™-Select HLB
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Sample preparations, Separation, Solid phase extractions, Solvents

Phospholipid Extractions for Cleanup or Enrichment

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. Santasania, An Trinh, and Mati Sarker
Reporter Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE. In other words, it may be difficult for a reversed-phase method or bonded ch...
Keywords: Environmental, Evaporation, Gas chromatography, High performance liquid chromatography, Homogenization, Metabolites, Phase transitions, Solid phase extractions, Solvents

Supel-Select HLB SPE for Pharmaceutical Analysis

Within the last 5-10 years, hydrophilic polymer SPE phases have become the phase of choice when developing new extraction methods that recover, concentrate, and cleanup small molecules (e.g., pharmac...
Carmen T. SantasaniaAn Trinh andMati Sarker
Reporter US Volume 27.3
Keywords: Antibiotics, Cancer, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolic Pathways, Pharmaceutical, Solid phase extractions, Solvents

Supel™-Select Polymeric SPE

Supel-Select SPE phases are ideal for the solid phase extraction of a broad range of compounds from aqueous samples. While reversed-phase interactions dominate retention on the Supel-Select HLB, and ...
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase extractions

UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Sample preparations, Solid phase extractions

UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan™ C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion

From our library of Articles, Sigma-Aldrich presents UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan™ C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Mass spectrometry, Metabolites, Pharmaceutical, Sample preparations, Solid phase extractions

Related Content

SPE Cartridges (Tubes) - Phase Selection Guide

The SPE Tube Configuraton Guide will help you choose the appropriate bed weight and cartridge dimensions.
Keywords: Agriculture, Calorimetry, Environmental, Fractionation, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Pharmaceutical, Solid phase extractions

Solid Phase Extraction: Fundamentals and Recent Developments [Webinar]

Solid Phase Extraction (SPE) has become a popular technique for sample preparation due to its convenience, effectiveness, relatively low solvent usage and versatility. The focus of this webinar is on...
Keywords: Sample preparations, Solid phase extractions

Supel™-Select Polymeric SPE [Product Overview Video]

Supel-Select HLB and Ion-Exchange phases are ideal for efficient and highly reproducible solid phase extraction of a broad range of compounds from aqueous samples. Their use covers a range of applica...
Keywords: Solid phase extractions

Peer-Reviewed Papers
15

References

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