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577122-U Supelco

Titan C18 UHPLC Column, 1.9 micron

1.9 μm particle size, L × I.D. 5 cm × 2.1 mm

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, Titan C18 UHPLC Columns, 1.9 μm
Quality Level   100
feature   endcapped
mfr. no.   Titan
extent of labeling   13% carbon loading
parameter   18000 psi pressure (1241 bar)
  60 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   5 cm × 2.1 mm
matrix   monodisperse silica particle platform
matrix active group   C18 (octadecyl) phase
particle size   1.9 μm
pore size   0.76 cm3/g pore volume
  80 Å pore size
surface area   410 m2/g
pH range   2 - 8
suitability   suitable for L1 per USP
separation technique   reversed phase

Description

General description

Titan C18 UHPLC Column (1.9 μM, L × I.D. 5 cm × 2.1 mm):
• Delivers an efficiency > 250,000 N/m, comparable to leading UHPLC columns.
• Narrow particle size distribution translates to rugged, reliable performance.

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Legal Information

Titan is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
When are additional phases expected for Titan 1.9μm columns?
Additional Titan 1.9μmphases are being developed and will encompass all aspects of UHPLC requirements.
Are guard columns available forTitan 1.9 μm C18 columns with 2.1 mm ID?
Guard columns for Titan 1.9 μm C18 UHPLC columns with 2.1 mm ID are product 577127-U.This product is a pack of three guard cartridges with dimensions 2.1 X 5 mm.These cartridges require the use of the guard column holder, product 577133-U.
Can I use Titan 1.9 μm columns on HPLC systems?
Titan 1.9 μm columns are designed to be used only on UPLC or UHPLC systems.
Which UHPLC Instruments can be used with Titan?
Titan 1.9μm columns can be used on any UPLC or UHPLC System.
What is the best practice for long-term storage of Titan C18 1.9 μm columns?
Long-term storage of silica-based, reversed-phase columns is best in 100% acetonitrile. Columns may be safely stored for short periods (up to 3 or 4 days) in most common mobile phases. However, when using buffers, it is best to protect both the column and the HPLC equipment and remove any salts by flushing the column with the same mobile phase without the buffer (e.g., when using 60:40 ACN:buffer, flush the column with 60:40 ACN:H2O) to eliminate any danger of corrosion from the salts while providing rapid reequilibration of the column with the original mobile phase. Before storing the column, the end-fittings should be tightly sealed with the end-plugs that are provided with the column to prevent the packing from drying.
What solvents are compatible with Titan C18 1.9 μm columns?
Water and all common organic solvents are compatible with Titan C18 1.9 μm columns.
Can the Titan 1.9 μm columns be reversed for column cleaning?
Direction of flow is marked on the column label for optimum performance.Column operation with flow in the direction opposing the arrow may produce reduced performance.However, reversed flow may be used to attempt removal of inlet frit plugging or contamination.
What sets Titan UHPLC columns apart from other manufacturers' sub 2 micron particle UHPLC columns?
The patent pending Ecoporous manufacturing process, which is the basis for the Titan stationary phase, revolutionizes high purity silica production. The end result is a highly efficient column at a remarkable value.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Articles

Bioanalytical Microsampling using Biocompatible SPME and Benefits over Dried Blood Spots

There is growing interest in clinical and bioanalytical research fields toward microextraction or microsampling techniques for sample isolation, storage, and transportation. This interest comes from ...
Craig Aurand
Reporter US Volume 33.3
Keywords: Adsorption, Centrifugation, Chromatography, Clinical, Evaporation, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Precipitation, Reductions, Sample preparations, Solid phase microextractions, Solvents

Developing Robust UHPLC Methods for Clinical, Forensic and Bioanalytical Samples

HybridSPE-PLus plates remove endogenous phospholipids for consistently high analyte response compared to protein precipitation plates. Combined with Titan UHPLC columns, they provide a powerful analy...
Craig Aurand, Senior Application Chemist and Jennifer Claus, Product Manager
Reporter US Vol 32.1
Keywords: Deposition, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Precipitation, Sample preparations, Separation

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS. Here we evaluate the hydrolysis efficiency of β-gluc...
Jim Blasberg
Harkewal Singh, Kevin Ray Sigma-Aldrich, St. Louis, MO
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites

HPLC Analysis of Isocyanates on Titan™ C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Isocyanates on Titan™ C18
Keywords: Air Monitoring, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

HPLC Analysis of Sulfa Drugs on Titan™ C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sulfa Drugs on Titan™ C18
Keywords: Antibiotics, Chromatography, High performance liquid chromatography

HPLC Analysis of Water-Soluble Vitamins Using Titan C18 Column

Vitamins are important compounds and are essential for normal metabolism. They are naturally found in many foods and are often added to processed food products. Water soluble vitamins include such co...
Olga Shimelis
Reporter US Volume 33.1
Keywords: High performance liquid chromatography, Metabolism, Sample preparations, Separation, Vitamins

LC/MS (TOF) Analysis of Drugs and Their Glucuronide Metabolites in Urine on Titan™ C18 after Solid Phase Extraction (SPE) using Supel™-Select SCX, β-Glucuronidase Enzyme Digestion

From our library of Articles, Sigma-Aldrich presents LC/MS (TOF) Analysis of Drugs and Their Glucuronide Metabolites in Urine on Titan™ C18 after Solid Phase Extraction (SPE) using Supel™-Select SCX, β-Glucuronidase Enzyme Digestion
Keywords: Analgesics, Anticonvulsants, Chromatography, Clinical, Digestions, High performance liquid chromatography, Mass spectrometry, Metabolites, Sample preparations, Solid phase extractions

LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan™ C18, 5 cm Column

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan™ C18, 5 cm Column
Keywords: Chromatography, Clinical, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions, Solvents

LC/MS/MS Analysis of Interacting Cardiac Drugs Digoxin, Quinidine, Amiodarone and Verapamil on Titan™ C18

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Interacting Cardiac Drugs Digoxin, Quinidine, Amiodarone and Verapamil on Titan™ C18
Keywords: Antihypertensives, Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Precipitation, Solid phase extractions

Mycotoxin Analysis in Food and Feed: Detecting the Fungus Among Us

Mycotoxin production by various fungi on food and feed crops is an ever-present danger that requires regular and reliable analytical testing.  The ubiquitous presence of fungi in our fields, combined...
Keywords: Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Separation, Solid phase extractions, Solvents

UHPLC Analysis of B Vitamins in Vitamin Water on Titan™ C18 using UV Detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of B Vitamins in Vitamin Water on Titan™ C18 using UV Detection
Keywords: Anticonvulsants, Chromatography, Pharmaceutical, Vitamins

UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection
Keywords: Anticonvulsants, Chromatography, Pharmaceutical, Vitamins

UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Acetonitrile Mobile Phase

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Acetonitrile Mobile Phase
Keywords: Chromatography, Vitamins

UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Fat Soluble Vitamins on Titan™ C18, Methanol Mobile Phase
Keywords: Chromatography, Vitamins

UHPLC Analysis of Kathon on Titan™ C18 using MS Detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Kathon on Titan™ C18 using MS Detection
Keywords: Chromatography, Cosmetics, High performance liquid chromatography, Mass spectrometry

UHPLC Analysis of Taxanes Using A Titan C18 Column and UV Detection

Taxanes are diterpenes produced by the plants of the genus Taxus (yews), and are widely used as chemotherapy agents. Taxane agents include paclitaxel (Taxol) and docetaxel (Taxotere). The role of tax...
Keywords: Alzheimer Disease, Antitumor agents, Cancer, Chromatography, Clinical, Drug discovery, High performance liquid chromatography, Medicinal chemistry, Oncology, Pharmaceutical, Reductions, Separation

UHPLC Analysis of Verapamil and Metabolites on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Verapamil and Metabolites on Titan™ C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Solvents

UHPLC/MS Analysis of Antineoplastic and Antidepressant Drugs in Plasma on Titan™ C18 after SPE using HybridSPE®-PLus

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Antineoplastic and Antidepressant Drugs in Plasma on Titan™ C18 after SPE using HybridSPE®-PLus
Keywords: Antitumor agents, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Sample preparations, Solid phase extractions, Solvents

UHPLC/MS Analysis of Heroin and Related Compounds on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Heroin and Related Compounds on Titan™ C18
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Solvents

UHPLC/MS Analysis of Morphine and Metabolites on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Morphine and Metabolites on Titan™ C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Solvents

UHPLC/MS of Drugs and Metabolites in Urine following Enzymatic Hydrolysis

β-D-Glucuronide glucuronosohydrolase enzymes, commonly shortened to β-glucuronidase, play an important role in the analysis of biological fluids for the presence of metabolites for drug screening and...
Craig Aurand, Manager; Kristen Brown, Pennsylvania State University
Reporter US Volume 33.2
Keywords: Carbohydrate metabolism, Derivatizations, Digestions, Enzyme activity, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Metabolites, Sample preparations, Separation, Solid phase extractions, Solvents

UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan™ C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion

From our library of Articles, Sigma-Aldrich presents UHPLC/MS/MS Analysis of Benzodiazepines and Glucuronide Metabolites in Urine on Titan™ C18 after SPE using Supel™-Select HLB, β-Glucuronidase Enzyme Digestion
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Mass spectrometry, Metabolites, Pharmaceutical, Sample preparations, Solid phase extractions

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Peer-Reviewed Papers
15

References

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Description

Add to Cart

577127-U Titan C18 UHPLC Guard Cartridge, 1.9 micron, 1.9 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea
577133-U Titan UHPLC Guard Cartridge Holder, for use with Titan Guard Cartridges, pkg of 1 ea

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