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577124-U Supelco

Titan C18 UHPLC Column, 1.9 micron

1.9 μm particle size, L × I.D. 10 cm × 2.1 mm

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, Titan C18 UHPLC Columns, 1.9 μm
Quality Level   100
feature   endcapped
mfr. no.   Titan
extent of labeling   13% carbon loading
parameter   18000 psi pressure (1241 bar)
  60 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   10 cm × 2.1 mm
matrix   monodisperse silica particle platform
matrix active group   C18 (octadecyl) phase
particle size   1.9 μm
pore size   0.76 cm3/g pore volume
  80 Å pore size
surface area   410 m2/g
pH range   2 - 8
suitability   suitable for L1 per USP
separation technique   reversed phase

Description

Recommended products

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Legal Information

Titan is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

WGK Germany 
WGK 1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
When are additional phases expected for Titan 1.9μm columns?
Additional Titan 1.9μmphases are being developed and will encompass all aspects of UHPLC requirements.
Are guard columns available forTitan 1.9 μm C18 columns with 2.1 mm ID?
Guard columns for Titan 1.9 μm C18 UHPLC columns with 2.1 mm ID are product 577127-U.This product is a pack of three guard cartridges with dimensions 2.1 X 5 mm.These cartridges require the use of the guard column holder, product 577133-U.
Can I use Titan 1.9 μm columns on HPLC systems?
Titan 1.9 μm columns are designed to be used only on UPLC or UHPLC systems.
Which UHPLC Instruments can be used with Titan?
Titan 1.9μm columns can be used on any UPLC or UHPLC System.
What is the best practice for long-term storage of Titan C18 1.9 μm columns?
Long-term storage of silica-based, reversed-phase columns is best in 100% acetonitrile. Columns may be safely stored for short periods (up to 3 or 4 days) in most common mobile phases. However, when using buffers, it is best to protect both the column and the HPLC equipment and remove any salts by flushing the column with the same mobile phase without the buffer (e.g., when using 60:40 ACN:buffer, flush the column with 60:40 ACN:H2O) to eliminate any danger of corrosion from the salts while providing rapid reequilibration of the column with the original mobile phase. Before storing the column, the end-fittings should be tightly sealed with the end-plugs that are provided with the column to prevent the packing from drying.
What solvents are compatible with Titan C18 1.9 μm columns?
Water and all common organic solvents are compatible with Titan C18 1.9 μm columns.
Can the Titan 1.9 μm columns be reversed for column cleaning?
Direction of flow is marked on the column label for optimum performance.Column operation with flow in the direction opposing the arrow may produce reduced performance.However, reversed flow may be used to attempt removal of inlet frit plugging or contamination.
What sets Titan UHPLC columns apart from other manufacturers' sub 2 micron particle UHPLC columns?
The patent pending Ecoporous manufacturing process, which is the basis for the Titan stationary phase, revolutionizes high purity silica production. The end result is a highly efficient column at a remarkable value.
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Protocols & Articles

Articles

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Infant formula on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Infant formula on Titan™ C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Saponifications, Vitamins

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Milk on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Milk on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Vitamins

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18
Keywords: Mass spectrometry, Separation, Vitamins

An Alternative to the USP and EP Methods for the Analysis of Riboflavin (Vitamin B2)

An alternative to the cumbersome USP and EP methods using LC/MS/MS detection and a Titan C18 UHPLC column to identify riboflavin and its impurities is described here. This new method is also compatib...
Nicolas J. Hauser, Carmen T. Santasania
Reporter US Volume 33.3
Keywords: Chromatography, Degradations, Detection methods, Eliminations, High performance liquid chromatography, Mass spectrometry, Metabolism, Oxidations, Sonication, Ultraviolet-Visible spectroscopy, Vitamins

Analysis of Bisphenol A and Analogous Compounds in Infant Formula

Bisphenol A (BPA) monomer is widely used for manufacturing plastics and epoxies. Such plastic materials are used to line food containers and metal cans. The monomer is never fully reacted, and detect...
Olga I. Shimelis, Principal R&D Scientist; K. G. Espenschied R&D Technician; and Jennifer Claus, Non-Bio Sample Preparation Product Manager
Reporter US Volume 34.4
Keywords: Chromatography, Eliminations, High performance liquid chromatography, Mass spectrometry, Polymerization reactions, Sample preparations, Separation, Solid phase extractions, Solvents

Analysis of Human Plasma Lipids Using sub-2 μm C18 UHPLC Column with MS Detection

 Lipidomics is a branch of metabolomics that, through an in-depth characterization, investigates the structures, functions and dynamic changes of lipids in cells, tissues, or bodily fluids. Moreover,...
Luigi Mondello, Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute”, Department, University of Messina, viale Annunziata, 98168 Messina, Italy
Reporter US Volume 34.4
Keywords: Capillary electrophoresis, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolomics, Phase transitions, Sample preparations, Separation, Size-exclusion chromatography, Solvents

Analysis of NBOMe Hallucinogens from Urine

Supel™-Select HLB plates provided excellent analyte recovery of these difficult compounds from urine samples with subsequent LC/MS (TOF) analysis on Ascentis® Express Fused-Core® 2.0 µm C18 columns. ...
Craig Aurand, David S. Bell
Reporter US, Volume 33.3
Keywords: Forensic, High performance liquid chromatography, Mass spectrometry, Sample preparations, Separation, Solid phase extractions

Analysis of Vitamin D in Milk and Infant Formula using UHPLC/MS/MS per AOAC Method 2011.11

Fortification of milk, dairy products, infant and adult nutritionals with vitamin D is required in many countries to provide additional dietary vitamin D. The fortification of milk in the USA, for ex...
Olga Shimelis
Reporter US Volume 33.2
Keywords: Chromatography, Evaporation, High performance liquid chromatography, Mass spectrometry, Metabolites, Sample preparations, Saponifications, Separation, Solvents, Vitamins

Column Selection Study for Analysis of Multiple Mycotoxins by LC-MS/MS

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Titan is a trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Separations Technology, Inc.
Emily R. Barrey1, Olga I. Shimelis1, and Christine Dumas2
1Div. of Sigma-Aldrich, Bellefonte, PA 16823 USA, 2Sigma-Aldrich, St.Galen, Switzerland
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation

HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea
Keywords: Chromatography, Derivatizations, High performance liquid chromatography, Size-exclusion chromatography, Solid phase extractions

HPLC Analysis of Water-Soluble Vitamins Using Titan C18 Column

Vitamins are important compounds and are essential for normal metabolism. They are naturally found in many foods and are often added to processed food products. Water soluble vitamins include such co...
Olga Shimelis
Reporter US Volume 33.1
Keywords: High performance liquid chromatography, Metabolism, Sample preparations, Separation, Vitamins

LC/MS Analysis of Azithromycin on Titan™ C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Azithromycin on Titan™ C18
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Tryptamines on TITAN™ Silica UHPLC Column

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Tryptamines on TITAN™ Silica UHPLC Column
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan™ C18, 10 cm Column

From our library of Articles, Sigma-Aldrich presents LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan™ C18, 10 cm Column
Keywords: Chromatography, Clinical, High performance liquid chromatography, Hormones, Liquid chromatography mass spectrometry, Mass spectrometry, Solvents

LC/MS/MS of Digoxin and Digitoxin in Serum or Plasma on Titan™ C18 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of Digoxin and Digitoxin in Serum or Plasma on Titan™ C18 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Solid phase extractions, Solvents

LC/MS/MS of Digoxin and Digitoxin in Serum or Plasma on Titan™ C18 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects

From our library of Articles, Sigma-Aldrich presents LC/MS/MS of Digoxin and Digitoxin in Serum or Plasma on Titan™ C18 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects
Keywords: Chromatography, High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations, Separation, Solid phase extractions, Solvents

Mycotoxin Analysis in Food and Feed: Detecting the Fungus Among Us

Mycotoxin production by various fungi on food and feed crops is an ever-present danger that requires regular and reliable analytical testing.  The ubiquitous presence of fungi in our fields, combined...
Keywords: Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Separation, Solid phase extractions, Solvents

Rapid, Sensitive, and Quantitative LC/MS/MS Determination of Digitoxin and Digoxin in Plasma

Digitoxin and digoxin are cardiac glycosides derived from the digitalis purpurea (Foxglove) plant. They have been in use for centuries for treatment of various heart conditions. Because of their narr...
Xiaoning Lu, David S. Bell
Reporter US Volume 33.3
Keywords: High performance liquid chromatography, Immunoassay, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Precipitation, Sample preparations, Separation, Solvents

Selective Extraction and Low Level Quantification of the Mycotoxin Patulin from Organic Apple Puree

Patulin (Figure 1) is a mycotoxin produced by several genera of mold including blue Penicillium species. The toxin was discovered and characterized in the 1940’s during routine general research condu...
K. G. Espenschied, Olga Shimelis, Michael Ye, Jennifer Claus
Reporter US Volume 33.2
Keywords: Antibiotics, Centrifugation, Chromatography, Filtration, Gastrointestinal, Genetic, High performance liquid chromatography, Sample preparations, Solid phase extractions

UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Aflatoxins on Titan™ C18 with TFA Derivatization and Fluoresence Detection
Keywords: Chromatography, Derivatizations, Sample preparations

UHPLC Analysis of Antibiotics/Antimalarials on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Antibiotics/Antimalarials on Titan™ C18
Keywords: Antibiotics, Chromatography, Mass spectrometry

UHPLC Analysis of Atrazine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Atrazine on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pesticides

UHPLC Analysis of Bisphenol A and Analogous Compounds in Infant formula on Titan™ C18 with Fluorescence Detection after SPE using SupelMIP® Bisphenol A

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Bisphenol A and Analogous Compounds in Infant formula on Titan™ C18 with Fluorescence Detection after SPE using SupelMIP® Bisphenol A
Keywords: Chromatography, Solid phase extractions

UHPLC Analysis of Conjugated Estrogens on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Conjugated Estrogens on Titan™ C18
Keywords: Chromatography, Clinical, Solvents

UHPLC Analysis of Corticosteroids on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Corticosteroids on Titan™ C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Mass spectrometry

UHPLC Analysis of Desipramine and Imipramine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Desipramine and Imipramine on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Deuterated Isomers on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Deuterated Isomers on Titan™ C18
Keywords: Anticonvulsants, Chromatography

UHPLC Analysis of Formoterol on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Formoterol on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Galangin on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Galangin on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Losartan and Quinapril on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Losartan and Quinapril on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Methapyrilene and Tripelennamine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Methapyrilene and Tripelennamine on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Nevirapine and Imiquimod on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Nevirapine and Imiquimod on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Nifedipine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Nifedipine on Titan™ C18
Keywords: Antihypertensives, Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Patulin in Cloudy Apple Cider on Titan™ C18 following SPE using SupelMIP® SPE - Patulin

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Patulin in Cloudy Apple Cider on Titan™ C18 following SPE using SupelMIP® SPE - Patulin
Keywords: Centrifugation, Chromatography, Sample preparations, Separation, Solid phase extractions, Solvents, Sonication

UHPLC Analysis of Phenylbutazone on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Phenylbutazone on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of Riboflavin and Impurities on Titan™ C18 with MS Detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Riboflavin and Impurities on Titan™ C18 with MS Detection
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolism, Oxidations, Solvents, Sonication, Ultraviolet-Visible spectroscopy, Vitamins

UHPLC Analysis of Taxanes Using A Titan C18 Column and UV Detection

Taxanes are diterpenes produced by the plants of the genus Taxus (yews), and are widely used as chemotherapy agents. Taxane agents include paclitaxel (Taxol) and docetaxel (Taxotere). The role of tax...
Keywords: Alzheimer Disease, Antitumor agents, Cancer, Chromatography, Clinical, Drug discovery, High performance liquid chromatography, Medicinal chemistry, Oncology, Pharmaceutical, Reductions, Separation

UHPLC Analysis of Temsirolimus and Sirolimus on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Temsirolimus and Sirolimus on Titan™ C18
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Solvents

UHPLC Analysis of Temsirolimus on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Temsirolimus on Titan™ C18
Keywords: Chromatography, Pharmaceutical

UHPLC Analysis of p-Acetophenetide (Phenacetin) Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of p-Acetophenetide (Phenacetin) Titan™ C18
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Pharmaceutical

UHPLC Analysis of p-Coumaric Acid on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of p-Coumaric Acid on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical, Vitamins

UHPLC Gradient Analysis of Boswellia Herbal Supplement on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Gradient Analysis of Boswellia Herbal Supplement on Titan™ C18
Keywords: Chromatography, Vitamins

UHPLC analysis of Taxanes on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC analysis of Taxanes on Titan™ C18
Keywords: Chromatography, Pharmaceutical

UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan™ C18
Keywords: Chromatography, Mass spectrometry, Metabolites

UHPLC-MS Analysis of Spice Cannabinoids and Metabolites using Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC-MS Analysis of Spice Cannabinoids and Metabolites using Titan™ C18
Keywords: Chromatography, Mass spectrometry

UHPLC/MS Analysis of Barbiturates on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Barbiturates on Titan™ C18
Keywords: Anticonvulsants, Chromatography, Clinical, Forensic, Mass spectrometry, Pharmaceutical, Separation, Solvents

UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Spice Cannabinoid Metabolites in Urine on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Sample preparations, Solid phase extractions

UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Spice Cannabinoids on Titan™ C18
Keywords: Chromatography, Forensic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Separation, Solvents

UHPLC/MS Analysis of Steroids on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Steroids on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Pharmaceutical

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Peer-Reviewed Papers
15

References

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Description

Add to Cart

577127-U Titan C18 UHPLC Guard Cartridge, 1.9 micron, 1.9 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea
577133-U Titan UHPLC Guard Cartridge Holder, for use with Titan Guard Cartridges, pkg of 1 ea

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