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  • 581304-U - Ascentis® C18 HPLC Column

581304-U Supelco

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 2.1 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Analytical column, 5 Micron, Analytical/Chromatography, Ascentis C18, Ascentis HPLC Columns, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   25% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 2.1 mm
surface coverage   3.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for (Similar to Phenomenex Luna C18)
  suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase


General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

Recommended products

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

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Protocols & Articles


HPLC Analysis of Peptide Mix, Complex, on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptide Mix, Complex, on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography

Selectivity of Peptides on Amide Embedded Polar Group Phases Versus Alkyl Phases

It has been known that small molecule analytes with hydrogen bond donor moieties display enhanced retention on embeddedpolar group (EPG) phases(1, 2, 3). However, no such systematic study has been do...
Hillel Brandes and Patrick Myers
Reporter EU Volume 20
Keywords: Chromatography, High performance liquid chromatography, Separation, Substitutions

The Extraction of Amphetamine and Related Drugs using Molecularly Imprinted Polymer SPE

Amphetamine and amphetamine related drugs consist of a class of stimulants and hallucinogens used by students, athletes, and recreation drug users. Over the last 15 years, abuse of these drugs has be...
Christine Widstrand, Staffan Bergström, Anna-Karin Wihlborg1, An Trinh2
Reporter US Volume 26.1
Keywords: Clinical, Clinical Chemistry, Enzyme-linked immunosorbent assay, Forensic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Solid phase extractions

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


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