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  • 581323-U - Ascentis® C18 HPLC Column

581323-U Supelco

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Analytical column, 5 Micron, Analytical/Chromatography, Ascentis C18, Ascentis HPLC Columns, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   25% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   5 cm × 4.6 mm
surface coverage   3.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for (Similar to Phenomenex Luna C18)
  suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase


General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Alternative Selectivity of Amide-based Embedded Polar Group HPLC Stationary Phases – Role of Hydrogen Bonding

High-performance liquid chromatographic (HPLC) method development often commences using alkyl (C8, C18) stationary phases. The method development scientist will commonly adjust the mobile phase organ...
Dave Bell
Reporter EU Volume 24
Keywords: Atomic absorption spectroscopy, High performance liquid chromatography, Ligands, Separation, Solvation

HPLC Analysis of Fluorinated Corticosteroids on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fluorinated Corticosteroids on Ascentis® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, con...
Jacinth A. M. McKenzie, Wayne K. Way
Reporter EU Volume 26
Keywords: Absorption, Applications, Biochemistry, Cancer, Cosmetics, Degradations, Environmental, Fluorinations, Forensic, Halogenations, Help, High performance liquid chromatography, Mass spectrometry, Metabolites, Methods, Oxidations, Pharmaceutical, Separation, Substitutions

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers


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