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581324-U Supelco

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical column, 5 Micron, Analytical/Chromatography, Ascentis C18, Ascentis HPLC Columns, HPLC Columns, by Brand for Small Molecules,
Quality Level   100
feature   endcapped
mfr. no.   Ascentis
extent of labeling   25% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 4.6 mm
surface coverage   3.7 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for (Similar to Phenomenex Luna C18)
  suitable for L1 per USP
Featured Industry   Food and Beverages
separation technique   reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the storage solvent for Product 581324-U, Ascentis® C18 HPLC Column - 5 ?m particle size, L × I.D. 15 cm × 4.6 mm?
The storage solvent is a ratio of 40:60 water:acetonitrile for this column.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What flow rate should I use with Ascentis® ExpressHPLC Columns?
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Can Ascentis® Express HPLC Columns be used for LC-MS?
Express Fused-Core particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Alternative Selectivity of Amide-based Embedded Polar Group HPLC Stationary Phases – Role of Hydrogen Bonding

High-performance liquid chromatographic (HPLC) method development often commences using alkyl (C8, C18) stationary phases. The method development scientist will commonly adjust the mobile phase organ...
Dave Bell
Reporter EU Volume 24
Keywords: Atomic absorption spectroscopy, High performance liquid chromatography, Ligands, Separation, Solvation

Analysis of Difficult Polar Compounds using Sigma-Aldrich Ion Pair Reagents and Supelco Ascentis HPLC Columns

Because reversed-phase HPLC is primarily dependent on hydrophobic interactions between the stationary phase and analyte, ion pairing is occasionally necessary to obtain sufficient retention of polar,...
Hansjörg Tinner
AnalytiX Volume 6 Article 4
Keywords: Absorption, Antibiotics, Degradations, High performance liquid chromatography, Phase transitions, Reversed-phase chromatography, Separation, Vitamins

HPLC Analysis of Analgesics on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Analgesics on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Anti-Inflammatory Drugs on Ascentis® C8

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Anti-Inflammatory Drugs on Ascentis® C8
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antibacterials/Antifungals on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibacterials/Antifungals on Ascentis® C18
Keywords: Antibiotics, Antifungals, Antimicrobials, Chromatography, High performance liquid chromatography, Pharmaceutical, Veterinary

HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® C18
Keywords: Antibiotics, Antimicrobials, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antibiotics on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibiotics on Ascentis® C18
Keywords: Antibiotics, Antimicrobials, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Anticholinergic Drugs on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Anticholinergic Drugs on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihistamines on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihistamines on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antihypertensive Drugs on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antihypertensive Drugs on Ascentis® C18
Keywords: Antihypertensives, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antineoplastic Drugs on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antineoplastic Drugs on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Antiparkinsonian Drugs on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antiparkinsonian Drugs on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Barbiturates on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Barbiturates on Ascentis® C18
Keywords: Anticonvulsants, Chromatography, Clinical, Forensic, High performance liquid chromatography, Pharmaceutical, Separation, Solvents

HPLC Analysis of Benzoic Acid Derivatives on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Benzoic Acid Derivatives on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Calcium Channel Blockers on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Calcium Channel Blockers on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Codeine and Metabolite on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Codeine and Metabolite on Ascentis® C18
Keywords: Analgesics, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Diclofenac and Salicylic Acid on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Fentanyl Tranquilizer on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fentanyl Tranquilizer on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Nitrazepam Anticonvulsant Drugs on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Nitrazepam Anticonvulsant Drugs on Ascentis® C18
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis® C18
Keywords: Antibiotics, Antimicrobials, Chromatography, Clinical, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Phenylacetic Acid on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Phenylacetic Acid on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Serotonin Metabolites on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Serotonin Metabolites on Ascentis® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Hormones

HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 55:45)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 55:45)
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 60:40)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 60:40)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Sunscreens on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sunscreens on Ascentis® C18
Keywords: Chromatography, Cosmetics, High performance liquid chromatography

HPLC Analysis of Tryptophan Hydroxylase Inhibitors on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tryptophan Hydroxylase Inhibitors on Ascentis® C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Mass spectrometry

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Xanthines on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Xanthines on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography

Ion Pairing for Analysis of Phosphonate Compounds

Phosphonates are a class of compounds commonly used for therapeutic applications of antiviral drugs. These compounds can be difficult to analyze due to their highly polar nature and lack of UV active...
Craig Aurand
Reporter US Volume 26.4
Keywords: Antivirals, Applications, Chromatography, Derivatizations, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Separation

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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Peer-Reviewed Papers
15

References

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