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  • 581509-U - Ascentis® Si HPLC Column

581509-U Supelco

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 2.1 mm

  •  eCl@ss 32110501

  •  NACRES SB.52



Related Categories Analytical column, 5 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis Si, Chromatography,
Quality Level   100
feature   endcapped: no
mfr. no.   Ascentis
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   15 cm × 2.1 mm
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   silica phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 6
suitability   suitable for L3 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  normal phase


General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

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Protocols & Articles


Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

In many cases, an exact knowledge of protein quantities is required for protein chemistry applications. Amino acid analysis enables precise determination of protein quantities and provides detailed i...
Keywords: Amino acid analysis, Derivatizations, High performance liquid chromatography, Separation

HPLC Analysis of Amino Acids on Ascentis® Si (Silica), HILIC Mode

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Amino Acids on Ascentis® Si (Silica), HILIC Mode
Keywords: Chromatography, High performance liquid chromatography

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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