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  • 58201 - SUPELCOSIL LC-Diol HPLC Column

58201 Supelco


5 μm particle size, L × I.D. 25 cm × 4.6 mm


  •  eCl@ss 32110501



Related Categories Analytical column, 5 mcron, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC Columns, by USP Code, HPLC/UHPLC,
feature   endcapped: no
packaging   pkg of 1 ea
mfr. no.   SUPELCOSIL
extent of labeling   3.5% carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
surface coverage   surface coverage 3.8 μmol/m2
matrix   silica gel, spherical particle platform
matrix active group   diol phase
particle size   5 μm
pore size   120 Å pore size
surface area   170 m2/g
pH-range   2 - 7.5
suitability   suitable for L20 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  normal phase


General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the phase for Supelcosil LC-Diol HPLC column, Product 58201?
The SUPELCOSIL LC-DIOL phase is a glycidoxypropylsilyl.
Can Supelcosil LC-Diol HPLC column, Product 58201,be use with acid?
No. The SUPELCOSIL LC-DIOL is very susceptible to cleaving of the phase using acids, therefore we do not recommend using acids, not even 1% acetic acid.
Can Supelcosil LC-Diol HPLC column, Product 58201,be used in a reversed-phase and normal phase system?
Yes. Before moving from one mode to another, the column should be flushed thoroughly with IPA (isopropyl alcohol).
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles


HPLC Analysis of Nonionic Surfactants, 9-10 Ethoxy Groups (Octylphenol) on SUPELCOSIL™ LC-Diol

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Nonionic Surfactants, 9-10 Ethoxy Groups (Octylphenol) on SUPELCOSIL™ LC-Diol
Keywords: Chromatography, High performance liquid chromatography

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59559 SUPELCOSIL LC-Diol Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

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