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58201C30 Supelco

SUPELCOSIL LC-Diol HPLC Column

5 μm particle size, L × I.D. 25 cm × 3 mm

  •  eCl@ss 32110501

  •  NACRES SB.52

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Properties

Related Categories Analytical column, 5 mcron, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, LC-Diol,
Quality Level   100
feature   endcapped: no
mfr. no.   SUPELCOSIL
extent of labeling   3.5% carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 3 mm
surface coverage   surface coverage 3.8 μmol/m2
matrix   silica gel, spherical particle platform
matrix active group   diol phase
particle size   5 μm
pore size   120 Å pore size
surface area   170 m2/g
pH-range   2 - 7.5
suitability   suitable for L20 per USP
Featured Industry   Food and Beverages
separation technique   hydrophilic interaction (HILIC)
  normal phase

Description

General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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