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58295 Supelco


5 μm particle size, L × I.D. 25 cm × 4.6 mm

  •  eCl@ss 32110501



Related Categories 5 micron LC-Si silica Columns, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, LC-Si silica,
mfr. no.   SUPELCOSIL
parameter   ≤70 °C temp. limit
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
matrix   silica gel, spherical particle platform
matrix active group   silica phase
particle size   5 μm
pore size   120 Å pore size
surface area   170 m2/g
pH-range   2 - 7.5
suitability   suitable for L3 per USP
compatibility   mode of use normal phase HPLC
separation technique   hydrophilic interaction (HILIC)
  normal phase


General description

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Milli-Q® Water Purification Solutions
Protocols & Articles

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