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58298 Supelco

SUPELCOSIL LC-18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

  •  eCl@ss 32110501

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Properties

Related Categories Analytical column, 5 micron, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC, LC-18,
feature   endcapped
mfr. no.   SUPELCOSIL
extent of labeling   11.0% carbon loading
parameter   ≤70 °C temp. limit
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
surface coverage   surface coverage 3.1 μmol/m2
matrix   silica gel, spherical particle platform
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   120 Å
surface area   170 m2/g
pH range   2 - 7.5
suitability   suitable for L1 per USP
separation technique   reversed phase

Description

General description

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Application

SUPELCOSIL LC-18 HPLC Column may be used for the separation of:
• Anthocyanins, flavonols, and carbohydrates in Tropea red onions (Allium cepa L.) during their determination by high performance liquid chromatography (HPLC) as well as HPLC coupled to mass spectrometry (MS) equipped with electrospray ionization (ESI) source.
• Sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice samples during their determination by semipreparative reversed phase HPLC.
• Aflatoxin M1 in milk and cheese samples during its determination by HPLC with post-column derivatization and fluorescence detection.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

EPA Method TO-11/IP-6A and ASTM® D5197: HPLC Analysis of Aldehydes and Ketones on SUPELCOSIL™ LC-18 after Collection/Desorption using LpDNPH

From our library of Articles, Sigma-Aldrich presents EPA Method TO-11/IP-6A and ASTM® D5197: HPLC Analysis of Aldehydes and Ketones on SUPELCOSIL™ LC-18 after Collection/Desorption using LpDNPH
Keywords: Air Monitoring, Chromatography, High performance liquid chromatography, Sample preparations, Solid phase extractions

HPLC Analysis of Fatty Acid Methyl Esters (FAMES) on SUPELCOSIL™ LC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fatty Acid Methyl Esters (FAMES) on SUPELCOSIL™ LC-18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Metabolites of 7,12-Dimethylbenz[a]anthracene on SUPELCOSIL™ LC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Metabolites of 7,12-Dimethylbenz[a]anthracene on SUPELCOSIL™ LC-18
Keywords: Chromatography, Clinical, Environmental, Environmental Analysis, High performance liquid chromatography

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18
Keywords: Clinical, High performance liquid chromatography, Vitamins

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Peer-Reviewed Papers
15

References

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