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59132 Supelco

SUPELCOSIL LC-NH2-NP HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Synonym: SUPELCOSIL LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN

  •  eCl@ss 32110501

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Properties

Related Categories Amino HPLC Columns, Analytical Column, 5 Micron, Analytical/Chromatography, Dedicated Normal Phase Amino, HPLC Columns, by Brand for Small Molecules,
feature   endcapped
mfr. no.   SUPELCOSIL
extent of labeling   3% Carbon loading
parameter   ≤70 °C temp. range
  400 bar pressure (5801 psi)
application(s)   HPLC: suitable
L × I.D.   25 cm × 4.6 mm
surface coverage   surface coverage 5.1 μmol/m2
matrix   silica gel, spherical particle platform
matrix active group   amino phase
particle size   5 μm
pore size   120 Å
surface area   170 m2/g
pH range   2 - 7.5
suitability   suitable for L8 per USP
Featured Industry   Food and Beverages
separation technique   normal phase

Description

General description

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.

These columns should be used with non-aqueous mobile phases only.

SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.

Features and Benefits

• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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59515 SUPELCOSIL LC-NH2-NP Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

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