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59564 Supelco

SUPELCOSIL LC-18 Supelguard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm

  •  eCl@ss 32110501



Related Categories Amino Acid Separations, Analytical Column, Analytical/Chromatography, HPLC Columns, by Brand for Small Molecules, HPLC/UHPLC,
Quality Level   100
packaging   pkg of 2 ea
parameter   <70 °C temp. range
application(s)   HPLC: suitable
L × I.D.   2 cm × 4 mm
matrix   silica gel high purity, spherical
matrix active group   C18 (octadecyl) phase
particle size   5 μm
pore size   120 Å
pH-range   2 - 7.5
suitability   suitable for L1 per USP
Featured Industry   Food and Beverages
compatibility   use to protect LC-18, LC-PAH
separation technique   reversed phase


General description

Guard columns are placed before the analytical column to protect and prevent harmful materials entering into the latter. It also helps as a backup to the in-line filter and also checks for leak through filter. The most suitable are short cartridge which is inserted into the cartridge holder.


SUPELCOSIL LC-18 Supelguard Cartridge was used as guard column for Supelcosil LC-18 HPLC column to analyze glucose tetrasaccharides in blood and urine samples using HPLC-electrospray ionization mass spectrometry (HPLC-ESI-MS). It was also suitable for analysing polar compounds in non-polar matrices using gas chromatography separation.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Supelguard is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

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Certificate of Analysis (COA)

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Protocols & Articles


Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

In many cases, an exact knowledge of protein quantities is required for protein chemistry applications. Amino acid analysis enables precise determination of protein quantities and provides detailed i...
Keywords: Amino acid analysis, Derivatizations, High performance liquid chromatography, Separation

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