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65603-U Supelco

Discovery® BIO Wide Pore C18 HPLC Column

3 μm particle size, L × I.D. 5 cm × 0.18 mm

  •  eCl@ss 32110501



Related Categories 3 micron Discovery BIO Wide Pore (300) C18 Columns, 3 micron Discovery Wide Pore C18 HPLC Columns, Analytical/Chromatography, C18 HPLC Columns, C18 for Biopolymers,
feature   endcapped
extent of labeling   9.2% Carbon loading
application(s)   HPLC: suitable
L × I.D.   5 cm × 0.18 mm
surface coverage   3.6 μmol/m2
matrix   silica particle platform
matrix active group   C18 (octadecyl) phase
particle size   3 μm
pore size   300 Å
surface area   100 m2/g
suitability   suitable for L1 per USP
mode of chromatography   reversed phase


General description

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

LC-MS Grade Solvents and Reagents

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles

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