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66825-U Supelco

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, BIOshell A400 Protein C4, 3.4 μm HPLC Columns, BIOshell HPLC Columns, C4, 3.4 μm Columns, HPLC & FPLC Columns for Biopolymer Separations,
Quality Level   100
material   stainless steel hardware
description   Shell thickness (0.2 μm)
  Solid Core (3.0 μm)
feature   endcapped
mfr. no.   BIOshell
extent of labeling   0.4% carbon loading
parameter   600 bar max. pressure
  90 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   10 cm × 2.1 mm
surface coverage   4.2 μmol/m2
matrix   spherical silica particle platform
matrix active group   C4 (butyl) phase
particle size   3.4 μm
pore size   400 Å
surface area   15 m2/g
operating pH range   2 - 9
suitability   suitable for L26 per USP
separation technique   reversed phase

Description

General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

A Fast and Reliable Method for Purity Analysis of Filgrastim

In recent years, there has been a large increase in the development of biological-based therapeutics, known as biologics, to treat a myriad of diseases. In 2015, biologics comprised 20% of the pharma...
Cory E. Muraco
Reporter US Volume 34.2
Keywords: Chromatography, Diseases, High performance liquid chromatography, Oxidations, Pharmaceutical, Separation

BIOshell™ A400 Protein C4 U/HPLC Columns

The BIOshell™ A400 Protein C4 column is a high-speed, high-performance liquid chromatography column based on a wide-pore (400 Å) Fused-Core® particle design.
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

Monoclonal antibodies (mAbs) are widely manufactured by many biopharmaceutical companies to treat a myriad of diseases ranging from Alzheimer’s disease, Parkinson’s disease, ulcerative colitis, and m...
Keywords: Amplification, Chromatography, Diffusion, Diseases, High performance liquid chromatography, Mass spectrometry, Methylations, Microscopy, Oxidations, Pesticides, Pharmaceutical, Phosphorylations, Reversed-phase chromatography, Scanning electron microscopy, Solid phase peptide synthesis

Fast UHPLC Analysis for IdeS Fragments of SiLu™Lite SigmaMAb Universal Antibody Standard on BIOshell A400 Protein C4

Both top-down and bottom-up approaches to protein characterization have their advantages and limitations. One such limitation of the top-down approach, particularly with large proteins, is the need f...
Hillel Brandes, and Stacy Shollenberger
Reporter US Volume 34.1
Keywords: Chromatography, Mass spectrometry, Reductions, Reversed-phase chromatography, Separation

Fast UHPLC Analysis of SILu™ Lite SigmaMAb Universal Antibody Standard IdeS Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents Fast UHPLC Analysis of SILu™ Lite SigmaMAb Universal Antibody Standard IdeS Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, Immobilization, Mass spectrometry, Reductions, Reversed-phase chromatography, Sample preparations, Separation

Faster Protein and Peptide Liquid Chromatography (FP2LC)

A new and broad product line for the analysis of peptides and proteins by reversed-phase high performance liquid chromatography (RP-HPLC) is introduced under the name BIOshell™ Fused-Core® U/HPLC col...
Roy Eksteen, and Hillel Brandes
Reporter US Volume 32.1
Keywords: Chromatography, High performance liquid chromatography, Ligands, Mass spectrometry, Reductions, Reversed-phase chromatography, Separation

HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Caseins on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography, Separation

HPLC Analysis of Humira on BIOshell™ A400 Protein C4 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Humira on BIOshell™ A400 Protein C4 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Native and Oxidized Filgrastim on BIOshell™ A400 Protein C4 Column
Keywords: Chromatography, Genetic, High performance liquid chromatography, Oxidations

HPLC Analysis of Oxidized SILu Lite SigmaMAb Universal Antibody Standard on BIOshell A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Oxidized SILu Lite SigmaMAb Universal Antibody Standard on BIOshell A400 Protein C4
Keywords: Chromatography, Oxidations, Reversed-phase chromatography, Separation

HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

HPLC Analysis of Reduced Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Reduced Monoclonal Antibody Fragments on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reductions

HPLC Analysis of SILu Lite Antibody Standard on BIOshell™ A400 Protein C4 Versus Competitor Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of SILu Lite Antibody Standard on BIOshell™ A400 Protein C4 Versus Competitor Column
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of an Oxidized Monoclonal Antibody Standard on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

HPLC of monoclonal antibody (mAb) SigmaMab on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC of monoclonal antibody (mAb) SigmaMab on BIOshell™ A400 Protein C4
Keywords: High performance liquid chromatography, Separation

UHPLC Analyses of Reduced Infliximab on BIOshell™ IgG 1000 Ã… C4 and BIOshell A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analyses of Reduced Infliximab on BIOshell™ IgG 1000 Ã… C4 and BIOshell A400 Protein C4
Keywords: High performance liquid chromatography, Pharmaceutical, Reductions

UHPLC Analysis of IgA from Human Serum on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of IgA from Human Serum on BIOshell™ A400 Protein C4
Keywords: Anti-inflammatory agents, Chromatography

UHPLC Analysis of IgM from Human Serum on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of IgM from Human Serum on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

UHPLC Analysis of Intact Infliximab on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Intact Infliximab on BIOshell™ A400 Protein C4
Keywords: Diseases, Pharmaceutical

UHPLC Analysis of a Rabbit Secretory IgA Monoclonal Antibody on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of a Rabbit Secretory IgA Monoclonal Antibody on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography

UHPLC/MS Analysis of Oxidized Filgrastim Variants on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Oxidized Filgrastim Variants on BIOshell™ A400 Protein C4
Keywords: Chromatography, Genetic, Mass spectrometry, Oxidations

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66830-U BIOshell A400 Protein C4, 3.4 μm Guard Cartridge, 3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

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