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66905-U Supelco

BIOshell A160 Peptide C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

  •  NACRES SB.52

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Properties

Related Categories Analytical/Chromatography, BIOshell A160 Peptide C18, 2.7 μm HPLC Columns, BIOshell HPLC Columns, C18, 2.7 μm Columns, HPLC & FPLC Columns for Biopolymer Separations,
Quality Level   100
material   stainless steel hardware
description   Shell thickness (0.5 μm)
  Solid Core (1.7 μm)
feature   endcapped: no
analyte chemical class(es)   peptides
mfr. no.   BIOshell
extent of labeling   4.6% carbon loading
parameter   600 bar max. pressure
  90 °C max. temp.
application(s)   HPLC: suitable
  UHPLC: suitable
L × I.D.   15 cm × 2.1 mm
surface coverage   2.2 μmol/m2
matrix   spherical silica particle platform
matrix active group   C18 (octadecyl) bonding phase, diisobutyloctadecyl
particle size   2.7 μm
pore size   160 Å
surface area   90 m2/g
operating pH range   1 - 8
suitability   suitable for L1 per USP
separation technique   reversed phase

Description

General description

BIOshell 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins. These columns contain advanced Fused-Core particles with pores strategically sized to 160 Å to enable optimized peptide diffusion. This attribute makes these columns an excellent choice for peptide mapping. Additionally, the sterically-protected C18 ligands provide extra stability allowing the columns to be used at an extended pH range (2-9) and high temperatures (up to 90 °C). This greatly expands the application range for the separation of biomolecules.

Application

UHPLC Analysis of Peptides on BIOshell A160 Peptide C18, CN, and Phenyl-Hexyl, 2.7 μm

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

HPLC Analysis of Insulin (Species Variants) on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Insulin (Species Variants) on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography, Separation

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell™ A160 2.7 μm Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell™ A160 2.7 μm Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell™ A160 Peptide C18, 2.7 μm
Keywords: Alkylations, Chromatography, High performance liquid chromatography, Nucleic acid denaturation, Sample preparations

Reversed-Phase HPLC Analysis of Insulin Variants and Analogs by UV and Mass Spectral Detection

Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recomb...
Cory Muraco, Stacy Shollenberger, and Hillel Brandes
MilliporeSigma, Bellefonte, PA USA
Keywords: Chromatography, High performance liquid chromatography, Hormones, Mass spectrometry, Pharmaceutical, Phase transitions, Reversed-phase chromatography, Separation

UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18 Using Ammonium Formate as a Mobile Phase Modifier

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18 Using Ammonium Formate as a Mobile Phase Modifier
Keywords: Chromatography, Mass spectrometry, Phase transitions, Separation

UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18 Using Difluoroacetic Acid as a Mobile Phase Modifier

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Insulin Variants and Analogs on BIOshell™ A160 Peptide C18 Using Difluoroacetic Acid as a Mobile Phase Modifier
Keywords: Chromatography, Mass spectrometry, Separation

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66918-U BIOshell A160 Peptide C18, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

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