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67032-U Supelco

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 15 cm × 75 μm

  •  NACRES SB.52



Related Categories Analytical/Chromatography, BIOshell A400 Protein C4, 3.4 μm HPLC Columns, BIOshell HPLC Columns, C4 for Biopolymers, C4, 3.4 μm Capillary Columns,
material   stainless steel hardware
description   Shell thickness (0.2 μm)
  Solid Core (3.0 μm)
feature   endcapped
mfr. no.   BIOshell
extent of labeling   0.4% carbon loading
parameter   600 bar max. pressure
  90 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   15 cm × 75 μm
surface coverage   4.2 μmol/m2
matrix   spherical silica particle platform
matrix active group   C4 (butyl) phase
particle size   3.4 μm
pore size   400 Å
surface area   15 m2/g
operating pH range   2 - 9
suitability   suitable for L26 per USP
separation technique   reversed phase


General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

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Protocols & Articles


BIOshell™ A400 Protein C4 U/HPLC Columns

The BIOshell™ A400 Protein C4 column is a high-speed, high-performance liquid chromatography column based on a wide-pore (400 Å) Fused-Core® particle design.
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

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