• USA Home
  • 67032-U - BIOshell A400 Protein C4, 3.4 μm HPLC Column

EMAIL THIS PAGE TO A FRIEND
67032-U Supelco

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 15 cm × 75 μm

  •  NACRES SB.52

Purchase

Properties

Related Categories Analytical/Chromatography, BIOshell A400 Protein C4, 3.4 μm HPLC Columns, BIOshell HPLC Columns, C4 for Biopolymers, C4, 3.4 μm Capillary Columns,
Quality Level   100
material   stainless steel hardware
description   Shell thickness (0.2 μm)
  Solid Core (3.0 μm)
feature   endcapped
mfr. no.   BIOshell
extent of labeling   0.4% carbon loading
parameter   600 bar max. pressure
  90 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   15 cm × 75 μm
surface coverage   4.2 μmol/m2
matrix   spherical silica particle platform
matrix active group   C4 (butyl) phase
particle size   3.4 μm
pore size   400 Å
surface area   15 m2/g
operating pH range   2 - 9
suitability   suitable for L26 per USP
separation technique   reversed phase

Description

General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

BIOshell™ A400 Protein C4 U/HPLC Columns

The BIOshell™ A400 Protein C4 column is a high-speed, high-performance liquid chromatography column based on a wide-pore (400 Å) Fused-Core® particle design.
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Separation

Related Content

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

Related Products

related product

Product #

Description

Add to Cart

00922 Difluoroacetic acid, for LC-MS LiChropur, ≥97.5% (GC)

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?