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  • 67490-U - BIOshell A400 Protein C18 Column

67490-U Supelco

BIOshell A400 Protein C18 Column

L × I.D. 15 cm × 75 μm, 3.4 μm particle size

  •  NACRES SB.52



Related Categories Analytical/Chromatography, BIOshell A400 Protein C18, 3.4 μm HPLC Columns, BIOshell HPLC Columns, C18 for Biopolymers, C18, 3.4 μm HPLC Columns,
Quality Level   100
material   stainless steel hardware (column)
description   Shell Thickness (0.2 μm)
  Solid Core (3.0 μm)
feature   endcapped
analyte chemical class(es)   proteins
mfr. no.   BIOshell
parameter   600 bar max. pressure
  90 °C max. temp.
application(s)   HPLC: suitable
L × I.D.   15 cm × 75 μm
matrix   spherical silica particle platform
matrix active group   C18 (octadecyl) phase
particle size   3.4 μm
pore size   3.4 μm particle size
  400 Å
surface area   ~15 m2/g
operating pH   1 - 9
suitability   suitable for HPLC
  suitable for L1 per USP
separation technique   reversed phase


General description

BIOshell 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.


UHPLC Analysis of Proteins on BIOshell A160 Peptide C18 2.7 μm; BIOshell A400 Protein C18, 3.4 μm; and BIOshell IgG 1000 Å C18, 2.7 μm

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Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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