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TSKgel® Phenyl-5PW RP HPLC Column

phase phenyl, L × I.D. 7.5 cm × 4.6 mm, 10 μm particle size

Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)



Related Categories Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories, TSKgel Phenyl-5PW RP Columns (1000 Å pores),
material   stainless steel
mfr. no.   Tosoh Phenyl-5PW, 08043
extent of labeling   1 meq/mL ligand density (estimate)
parameter   no limitation salt concentration
  0-100% organic solvent
  1.2 mL/min flow rate
  30 bar max. pressure
  4-60 °C temperature
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 4.6 mm
matrix   polymer particle platform
matrix active group   phenyl phase
particle size   10 μm
pore size   1000 Å pore diameter
operating pH   2 - 12
separation technique   reversed phase


General description

TSKgel Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography. In contrast, TSKgel Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 A pore size accommodates globular protein samples up to 1,000,000 Da.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Safety & Documentation

Safety Information

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Protocols & Articles

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819007 TSKgel® Phenyl-5PW RP Guard Cartridge, phase phenyl, L × I.D. 1.5 cm × 3.2 mm, 10 μm particle size, pkg of 3 ea

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