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TSKgel® Ether-5PW HPLC Column

phase ether, L × I.D. 7.5 cm × 8 mm, 10 μm particle size



Related Categories Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Hydrophobic Interaction (HIC) Columns, TSKgel Ether-5PW Column (1000 Å pore size),
material   glass
mfr. no.   Tosoh, 14014
parameter   ≤4 M salt concentration
  ≤50% organic solvent
  1.2 mL/min flow rate
  10-50 °C temperature
  20 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 8 mm
matrix   hydroxylated methacrylic polymer
matrix active group   ether phase
particle size   10 μm
operating pH   2 - 12
separation technique   hydrophobic interaction (HIC)


General description

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

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TSKgel is a registered trademark of Tosoh Corporation

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Protocols & Articles


HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Mouse Monoclonal Antibodies (MAb) on TSKgel® Ether-5PW
Keywords: Chromatography, High performance liquid chromatography

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814025 TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 1 cm × 8 mm, 20 μm particle size, glass

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