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TSKgel® Phenyl-5PW HPLC Column

phase phenyl, L × I.D. 7.5 cm × 2 mm, 10 μm particle size, stainless steel hardware

Synonym: TSKgel® Hydrophobic Interaction Chromatography (HIC) Column

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, HPLC & FPLC Columns for Biopolymer Separations, HPLC/UHPLC, Hydrophobic Interaction (HIC) Columns, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories,
material   stainless steel hardware
mfr. no.   Tosoh Phenyl-5PW, 18759
parameter   ≤4 M salt concentration
  ≤50% organic solvent
  0.12 mL/min flow rate
  10-50 °C temperature
  8 bar max. pressure
application(s)   HPLC: suitable
L × I.D.   7.5 cm × 2 mm
matrix   polymer particle platform
matrix active group   phenyl phase
particle size   10 μm
pore size   1000 Å
operating pH   2 - 12
separation technique   hydrophobic interaction (HIC)


General description

The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients. Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.

TSKgel BioAssist Phenyl columns contain the same particles as Phenyl-5PW columns, but are packed in PEEK column hardware rather than stainless steel. BioAsist Phenyl (Phenyl-5PW), TSKgel Ether-5PW and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing, such as BioAssist Phenyl. We recommend an Ether-5PW column for purifying very hydrophobic proteins. TSKgel BioAssist Phenyl and TSKgel Ether-5PW packings are based on TSKgel G5000PW resin - 10 μm particles with 1000 Å pores. TSKgel Butyl-NPR columns, which are less hydrophobic than Ether-5PW, is prepared from 2.5 μm nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Protocols & Articles


HPLC Analysis of Large RNAs on TSKgel® Phenyl-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Large RNAs on TSKgel® Phenyl-5PW
Keywords: Chromatography, High performance liquid chromatography

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